Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-771-9 | CAS number: 150-78-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 14 nov 1995 to 15 feb 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,4-dimethoxybenzene
- EC Number:
- 205-771-9
- EC Name:
- 1,4-dimethoxybenzene
- Cas Number:
- 150-78-7
- Molecular formula:
- C8H10O2
- IUPAC Name:
- 1,4-dimethoxybenzene
- Details on test material:
- - Name of test material (as cited in study report): Hydrochinondimethylether
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Tierzucht Scönwalde GmbH i.G., Hauptstrasse 62, 16352 Schönwalde
- Age at study initiation: 7 weeks
- Weight at study initiation: males: mean 34.4 g; females: mean 28.1 g
- Assigned to test groups randomly: yes, under following basis: randomisation schemes 95.0790 and 95.0791
- Fasting period before study: no data
- Housing: in makrolon cages Type 3, five animals per cage on soft wood granulate
- Diet: rat/mice diet ssniff R/M-H (V 1534), ad libitum. ssniff GmbH, Postbox 2039, 59480 Soest.
- Water: tap water in plastic bottles ad libitum
- Acclimation period: 1 week under study conditions
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3°C
- Humidity (%): 50 +/- 20%
- Air changes (per hr): in fully air-conditioned room.
- Photoperiod (hrs dark / hrs light): 12h / 12h daily
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: starch mucilage at the appropriate concentration.
- Details on exposure:
- no data
- Duration of treatment / exposure:
- Once orally.
- Frequency of treatment:
- 1 exposure
- Post exposure period:
- None
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0; 2000 mg/kg bw
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5 males and 5 females per dose.
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- Endoxan R containing cyclophosphamide, soluted in distilled water.
- Route of administration: oral, gavage
- Doses / concentrations: 50 mg/kg bw
Examinations
- Tissues and cell types examined:
- Bone marrow smears for the occurence of micronuclei in red blood cells were examined.
Cell types examined: polychromatic and normochromatic erythrocytes. - Details of tissue and slide preparation:
- The animals were killed by carbon dioxide asphyxiation 12, 24 or 48 hours after administration. For each animal, about 3 ml foetal bovine serum was poured into a centrifuge tube. Both femora were removed and the bones freeds of muscle tissue. The proximal ends of the femora were opened and the bone marrow flushed into the centrifuge tube. A suspension was formed. The mixture was then centrifuged for 5 minutes at approximlately 1200 rpm, after which almost all the supernantant was discarded. One drop of the thoroughly mixed sediment was smeared onto a clean slide, identified by project code and animal number and air-dried for about 12 hours.
- Evaluation criteria:
- 1000 polychromatic erythrocytes were counted for each animal. The number of cells with micronuclei was recorded, not the number of individual micronuclei. As a control measure 1000 mature erythrocytes were also counted and examined for micronuclei.
In addition, the ratio of polychromatic to normochromatic erythrocytes was determined. All bone marrow smears for evaluation were coded to ensure that the group from which they were taken remained unknown to the investigator. The number of polychromatic erythrocytes with micronuclei occuring in the 1000 polychromatic erythrocytes, and the number of normocytes with micronuclei occuring in the 1000 normocytes were evaluated statistically. - Statistics:
- Wilcoxon-Test.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
- Dose range: 2000 mg/kg bw
- number of animals: 3 males and 3 females.
- Clinical signs of toxicity in test animals: none
- mortality: none
- Macroscopic finding: no macroscopic findings were observed
Applicant's summary and conclusion
- Conclusions:
Paradimethoxybenzene is not mutagenic in the micronucleus test in male and female NMRI mice.- Executive summary:
The micronucleus test was performed with Paradimathoxybenzene (Hoechst, 1996), according to the OECD 474 guideline under GLP conditions.
The test compound was suspended in starch mucilage and was given once as an orally dose of 2000 mg/kg bw to male and female NMRI mice, based on the results of a preliminary study.
According to the test procedure, the animals were killed 12, 24 or 48 hours after administration.
Endoxan (cyclophosphamide in distilled water) was used as positive control substance and was administered once orally at a dose of 50 mg/kg bw.
The number of polychromatic and normochromatic erythrocytes containing micronuclei was not increased. The ration of polychromatic/normochromatic erythrocytes in both male and female animals remained unaffected by the treatment with Paradimethoxybenzene and was statistically not different from the control values.
Positive control induced in both males and females a marked statistically significant increase in the number of polychromatic cells with micronuclei, indicating the sensitivity of the test system. The ratio of polychromatic erythrocytes to normocytes was not changed to a significant extent.
Under the test conditions, the results indicate that Paradimethoxybenzene is not mutagenic in the micronucleus test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.