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Diss Factsheets
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EC number: 221-043-3 | CAS number: 2983-37-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 2: Semi-static method)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1984
- GLP compliance:
- yes
Test material
- Reference substance name:
- Ethyl 2-ethylhexanoate
- EC Number:
- 221-043-3
- EC Name:
- Ethyl 2-ethylhexanoate
- Cas Number:
- 2983-37-1
- Molecular formula:
- C10H20O2
- IUPAC Name:
- ethyl 2-ethylhexanoate
- Details on test material:
- - Name of test material (as cited in study report): 2-Ethyl-ethylcapronat
- Physical state: liquid
- Analytical purity: 100%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100, 300 mg/L
- Sampling method: not described in test report
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- due to low solubility in water, the test substance was directly weighed into the test vessels and treated 10 s with ultraturrax
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Westaquarium
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Weight at study initiation (mean and range, SD): no data
- Feeding during test
- Food type: pelleted Altromin 1324
- Amount: no data
- Frequency: no data
ACCLIMATION
- Acclimation period: no data
- Acclimation conditions (same as test or not): no data
- Type and amount of food: pelleted Altromin 1324
- Feeding frequency:no data
- Health during acclimation (any mortality observed): mortality < 0.1% during the week before the test
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 22.0 - 23.5°C
- pH:
- 8.2 - 8.4
- Dissolved oxygen:
- 70 - 97 mg/L in 24 h old media
- Nominal and measured concentrations:
- nokinal: 0, 100, 300, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: aquaria with 5 L fill volume
- Aeration: no data
- Renewal rate of test solution: 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate:< no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
mortality: 0, 6, 24, 48, 72, 96 h - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 173 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- not applicable
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Cumulative Mortality:
Conc. [mg/L]
0 h
6 h
24 h
48 h
72 h
96 h
0
0
0
0
0
0
0
100
0
0
0
0
0
0
300
0
0
100
100
100
100
1000
0
100
100
100
100
100
As the test substance is stable in water and the oxygen concentration did not decrease drastically (i.e. no biodegradation occurred) , it was assumed that the substance was present in the test system in total (either dissolved, dispersed or deposed).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 of 2-Ethyl ethyl capronate in this acute toxicity test with Zebrafish was 173 mg/L.
- Executive summary:
In a 96-h acute toxicity study according to ISO 7346 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish - Part 2: Semi-static method), Zebrafish (Brachydanio rerio; new name: Danio rerio), were exposed to 2-Ethyl ethyl capronate (100% a.i.) at nominal concentrations of 0 (control), 100, 300 and 1000 mg/L under semi-static conditions.
No mortality was observed in the control and at 100 mg/L during the 96 h test period. At 300 mg/L 10/10 fish were dead after 24 h; at 1000 mg/L 10/10 fish died within 6 h.
The 96 h LC50 was 173 mg a.i./L.
Results Synopsis
Test organism: Zebrafish (Brachydanio rerio; new name: Danio rerio)
Test Type: Static Renewal
LC50: 173 mg a.i./L
Endpoint(s) Effected: mortality
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