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EC number: 248-053-0 | CAS number: 26850-47-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26.-28.11.1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Part I, 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- No data.
- Vehicle:
- no
- Details on test solutions:
- No data.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: n.a.
- Source: daphnids were bred in the laboratories of the TF Product Ecology under standardized conditions
- Age of parental stock (mean and range, SD): < 24 h - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 136 mg/L CaCO3
Alkalinity: 0.8 mmol/L - Test temperature:
- 21 °C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- Test media were saturated with oxygen at the beginning of the test.
- Salinity:
- no data
- Conductivity:
- no data
- Nominal and measured concentrations:
- nominal: 0.1, 0.18, 0.32, 0.58 and 1.0 ppm
measured mean concentration: 0.08, 0.14, 0.25, 0.45, 0.78 ppm - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 250 ml beakers containing 100 ml test medium
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): n.a.
- Renewal rate of test solution (frequency/flow rate): /
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: bidistilled water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Intervals of water quality measurement: at beginning and at end of the test
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility after 24 and 48 h
VEHICLE CONTROL PERFORMED: yes (blank control)
RANGE-FINDING STUDY
- Test concentrations: no details reported
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.17 other: ppm
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limit
- Remarks:
- 0.14 - 0.19
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.08 other: ppm
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 0.45 other: ppm
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Test evaluations were done according to the guidelines by visual assessment of the mobility of the daphnids after 24 and 48 hours.
The EC50 was calculated by using the Probit model and by graphical determination on logarithmic probability paper.
The concentration of the test substance at the beginning and at the end of the test was analysed by the analytical Service Klybeck. - Results with reference substance (positive control):
- EC50 (24 h) = 0.9 ppm (range: 0.8 - 1.5 ppm)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 of the test item to Daphnia magna under the conditions of the OECD test guideline 202 is 0.17 ppm.
- Executive summary:
The 48h acute toxicity of the test item to Daphnia magna was studied under static conditions. Daphnids were exposed to 0 (blank control), 0.08, 0.14, 0.25, 0.45, 0.78 ppm (measured concentration). Mortality/immobilisation were observed daily. The 48h EC50was 0.17 ppm. The 48h EC0 based on mortality/immobilisation was 0.08 ppm.
Based on the results of this study, the test item would be classified in category Chronic 1 in accordance with the classification system of the GHS.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age (e.g. 1stinstar): < 24 h
Test Type: Static
EC0: 0.08 ppm
EC50: 0.17 ppm 95% C.I.: 0.14 -0.19 ppm
Endpoint(s) Effected: mortality/immobilisation
Reference
1) Analytical results of test substance measurements:
Nominal concentration [ppm] |
Concentration start [ppm] |
Concentration end [ppm] |
Mean value* [ppm] |
0.10 |
0.08 |
0.07 |
0.08 |
0.18 |
0.14 |
0.13 |
0.14 |
0.32 |
0.26 |
0.23 |
0.25 |
0.58 |
0.47 |
0.42 |
0.45 |
1.00 |
0.80 |
0.75 |
0.78 |
* used for calculation of EC50 value
2) Oxygen concentration and pH
The oxygen concentration and the pH was controlled at the beginning and at the end of the test.
Start: - Oxygen: saturated
- pH: 8.0 (highest test concentration)
- Temp.: 21° C
End:
Nominal Conc. [ppm] |
0.1 |
0.18 |
0.32 |
0.58 |
1.0 |
Blanc |
Oxygen [%] |
96 |
96 |
96 |
97 |
96 |
97 |
pH |
7.9 |
7.8 |
7.9 |
7.9 |
7.9 |
7.7 |
Temp. [°C] |
21 |
21 |
21 |
21 |
21 |
21 |
3) Influence of the Test Item on the immobilisation of Daphnia magna:
Nominal concentration [ppm] |
No. of animals |
No. of responses |
Immobility [%]* |
||
24 h |
48 h |
24 h |
48 h |
||
0 (Blank) |
10 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
|||
0.08 |
10 |
0 |
0 |
0 |
0 |
10 |
0 |
0 |
|||
0.14 |
10 |
1 |
4 |
5 |
20 |
10 |
0 |
0 |
|||
0.25 |
10 |
9 |
9 |
95 |
95 |
10 |
10 |
10 |
|||
0.45 |
10 |
9 |
10 |
90 |
100 |
10 |
9 |
10 |
|||
0.78 |
10 |
10 |
10 |
100 |
100 |
10 |
10 |
10 |
* calculated for 20 animals
Description of key information
The 48h acute toxicity of the test item to Daphnia magna was studied under static conditions in accordance with OECD test guideline 202. Daphnids were exposed to 0 (blank control) and mean measured concentrations of 0.08, 0.14, 0.25, 0.45, 0.78 ppm. The 48h EC50 was 0.17 ppm.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.17 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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