1,3,5-Benzenetricarboxylic acid, copper(2+) salt (2:3)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24.04.2016 - 06.05.2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

1,3,5-Benzenetricarboxylic acid, copper(2+) salt (2:3)

Cas Number:

309721-49-1

Molecular formula:

C9 H6 O6 . 3/2 Cu

IUPAC Name:

1,3,5-Benzenetricarboxylic acid, copper(2+) salt (2:3)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Batch No.of test material: APP199.6

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

Origin:
Sammlung von Algenkulturen (SAG), Pflanzenphysiologisches Institut der Universität Göttingen, Nikolausberger Weg 18, D-37073 Göttingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

26.88 mg/L CaCO3

Test temperature:

Mean value: 23.2 °C

pH:

7.93 - 8.61

Conductivity:

154.4 µS/cm

Nominal and measured concentrations:

5 loadings were tested in a geometric series with a factor of approximately 2.2: 0.043 - 0.094 - 0.20 - 0.46 - 1.00 mg/L.
measured copper concentration at the start of exposure [µg/L]: 2.7, 5.0, 11, 31, 53

Details on test conditions:

For each loading level a stock dispersion was prepared with dilution water (without EDTA) (see table 3) according to the nominal loadings of the test item. An appropriate amount of the test item was weighed out and transferred with an appropriate amount of the dilution water into a glass flask. The dispersions were stirred with a magnetic stirrer at approximately 1100 rpm for 24 hours at room temperature. After completion of stirring, undissolved test item was removed by membrane filtration (membrane filter 0.45 µm, RC, MACHEREY-NAGEL) to achieve the final test concentrations. During filtration the filter was always kept covered. The filter was saturated in order to avoid adsorption during the filtration. The first 25 mL filtrate were discarded. The filtration was interrupted for ca. 15 minutes to allow adsorption and saturation of the filter material with dissolved test item. Thereafter, the filtration was continued. The next 25 mL were discarded. The following filtrate was used in the test.
3 replicates per loading level and 6 for the control were tested.

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

0.173 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EL10

Effect conc.:

0.046 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate