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EC number: 224-924-0 | CAS number: 4553-89-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short
term toxicity to fish:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate
(UERL study report, Report no. 4553 -89 -3/01/2015/FT, Sustainability Support Services (Europe) AB has the letter of access, 2015). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system). The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio) were exposed to these concentration for 96 hours. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 value was determined to be > 100mg/L. Based on the above LC50 value it can be concluded that the target substance 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is non toxic to fish and thus can not be classified under Aquatic toxic category.
Short
term toxicity to aquatic invertebrate:
The
Lethal concentration (LC50) of the substance Disodium
4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di-
(naphthalene-1-sulfonate) in Daphnia magna in a 48 hour study is
estimated to be 145.22 mg/L.
Toxicity
to aquatic algae and cynobacteria:
After
72 hours of exposure to various nominal test concentrations of disodium
4,4’-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate
(CAS No. 4553-89-3) to freshwater green alga Chlorella vulgaris, EC50
was determined to be 379.668 mg/l graphically through probit analysis.
Toxicity
to microorganisms:
The
Effective concentration (LC50) of the substance Disodium
4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di-
(naphthalene-1-sulfonate) in Vibrio fischeri in a 30 minute study is
estimated to be 12.99 mg/L based on the effects on physiology.
Additional information
Short
term toxicity to fish:
Data available for short term toxicity to fish endpoint of the substance Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) are summarized as follows:
In key study, Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (UERL study report, Report no. 4553 -89 -3/01/2015/FT, Sustainability Support Services (Europe) AB has the letter of access, 2015). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system). The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio) were exposed to these concentration for 96 hours. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 value was determined to be > 100mg/L.
While in supporting study, Short term toxicity to fish of Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) is estimated using QSAR Toolbox version 3.3 (2015). The Lethal concentration (LC50) of the substance Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) in fish Oncorhynchus mykiss in a 96 hour study is estimated to be 1311.92 mg/L.
Based on the above LC50 values it can be concluded that the target substance 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is not likely to be toxic to fish and thus cannot be classified under Aquatic toxic category as per CLP regulation.
Short
term toxicity to aquatic invertebrate:
Short
term toxicity to aquatic invertebrates of Disodium
4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di-
(naphthalene-1-sulfonate) was estimated using QSAR Toolbox version 3.3.
The Lethal concentration (LC50) of the substance Disodium
4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di-
(naphthalene-1-sulfonate) in Daphnia magna in a 48 hour study is
estimated to be 145.22 mg/L. This value indicates that the substance is
not likely to exhibit toxicity to aquatic invertebrates as per the CLP
criteria.
Toxicity
to aquatic algae and cynobacteria:
Available
Experimental and predicted data of the target substance Disodium
4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di-
(naphthalene-1-sulfonate) for the Short term toxicity to aquatic
invertebrate are summarized as follows:
For key study, the effect of test item disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate, CAS No. 4553-89-3 was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentrations chosen for the study were 12.5mg/l, 25mg/l, 50 mg/l, 100mg/l, 200 mg/l and 400mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50, calculated graphically through probit analysis, was observed to be 379.668 mg/l.
Supporting above experimental study, toxicity to aquatic algae and cyanobacteria of Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) was estimated using QSAR Toolbox version 3.3. The Effective concentration (LC50) of the substance Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) in algae Pseudokirchneriella subcapitata in a 72 hour study is estimated to be 155.21 mg/L. based on the effects on population.
Based on the above LC50 values it can be concluded that the target substance 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is not likely to be toxic to algae and thus cannot be classified under Aquatic toxic category as per CLP criteria.
Toxicity
to microorganisms:
Toxicity
to micro-organisms of Disodium 4,4’-(2,4-dihydroxy-5-
hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) was
estimated using QSAR Toolbox version 3.3. The Effective concentration
(LC50) of the substance Disodium 4,4’-(2,4-dihydroxy-5-
hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) in
Vibrio fischeri in a 30 minute study is estimated to be 12.99 mg/L based
on the effects on physiology while in Pseudomonas putida in a 17 hour
study it is estimated to be 6597.21 mg/L based on growth inhibition
effect.
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