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EC number: 224-924-0 | CAS number: 4553-89-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard OECD test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Domestic waste water, surface soil and soil samples from polluted sites receiving predominantly domestic waste
- Details on inoculum:
- Domestic waste water, surface soil and soil samples from polluted sites receiving predominantly domestic waste was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions. This gave the bacterial count as107to 108CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- 1. Respirometer flasks were allowed to reach the desired temperature at 200C.The test item, i.e., disodium4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate, was taken at a concentration of 100mg/L in mineral medium. The reference item chosen was Sodium benzoate with a concentration of 100mg/L in the mineral medium was directly added to the Respirometer Flask according the volume of mineral media taken.
2. The Respirometer Flasks of test item & reference item were filled with 400ml and 250ml of mineral media, respectively. Test suspension volume was decided by considering the available information on biodegradability of that test item.
3. All the Respirometer Flasks were inoculated with prepared mixed inoculum at the concentration of 10ml/L. This concentration gave the bacterial CFU/ml as 107to 108.
4. The initial pH of the test medium was 7.4. The pH of all test vessels was measured at 28th day.
5. 2-4 pellets of KOH was added in the alkali holder, which acts as CO2 absorber.
6. The respirometric sensors containing the KOH pellets were tightly and carefully screwed on each of the Respirometer Flasks. The sensors were reset by deleting old recorded data and choosing the appropriate scale (The sensor gives the oxygen consumption values in mgO2/l).
7. Respirometer Flask were kept in the respirometer incubator at constant temperature of 20 ± 1°C for 28 days.
8. Every day oxygen consumption (mg O2/l) into the each bottle was recorded. Temperature and stirring condition were checked regularly. - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- concentration 100 mg/L
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 2.8
- Sampling time:
- 28 d
- Details on results:
- The BOD28 value of disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (CAS No. 4553-89-3) was observed to be 0.0385 mgO2/mg. ThoD was determined by calculation as 1.373 mgO2/mg. % Degradation was calculated using the values of BOD and ThOD for test item and was found to be 2.80 %. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was found to be 77.36%.
The mean BOD values (mg O2/l) in control on 28th day was 28.2 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). - Results with reference substance:
- The percentage degradation of reference substance (Sodium benzoate) has reached the pass levels (60% of its ThOD) by Day 14. Thus, fulfilling the validity criteria of the study.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (CAS No. 4553-89-3) undergoes 2.80 % biodegradation after 28 days in the test condition. Thus, the test item can be inferred/considered as not readily biodegradable.
- Executive summary:
28-days Manometric respirometry test following the OECD guideline 301F to determine the ready biodegradability of disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate(CAS No. 4553-89-3). The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item.
The BOD28value of disodium-4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (CAS No. 4553-89-3) was observed to be 0.0385 mgO2/mg. ThOD was calculatedas 1.373 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was found to be 2.80%. Based on the results, the test item, under the test conditions, was found to be not readily biodegradable at 20 ± 1°C over a period of 28 days.
Reference
TABLE 1: MEAN RESPIROMETRIC VALUES (oxygen consumed mg O2/l)
No. of Days |
Inoculum Blank (Control) |
Test Suspension |
Procedure Control (Reference Item) |
1 |
11.4 |
12 |
26.5 |
2 |
15.6 |
16.1 |
26.5 |
3 |
20 |
20.5 |
23 |
4 |
24.1 |
26.5 |
40 |
5 |
24.3 |
27.3 |
70.5 |
6 |
26.3 |
29.3 |
100.5 |
7 |
26.2 |
29.5 |
108 |
8 |
26.5 |
29.8 |
120.5 |
9 |
- |
- |
- |
10 |
- |
- |
- |
11 |
26.8 |
30.6 |
127 |
12 |
26.2 |
29.8 |
133 |
13 |
26.8 |
30.4 |
142.5 |
14 |
- |
- |
- |
15 |
27.1 |
30.6 |
144.5 |
16 |
27.1 |
30.9 |
146.5 |
17 |
- |
- |
- |
18 |
27.1 |
30.9 |
150 |
19 |
27.3 |
30.9 |
152 |
20 |
27.6 |
31.5 |
154.5 |
21 |
27.1 |
30.9 |
154.5 |
22 |
27.6 |
31.5 |
155.5 |
23 |
27.6 |
31.8 |
155 |
24 |
27.9 |
32.05 |
156.5 |
25 |
- |
- |
- |
26 |
27.6 |
31.8 |
157 |
27 |
28.2 |
32.05 |
158 |
28 |
28.2 |
32.05 |
157 |
TABLE 2: BOD values (mgO2/mg)
No. of Days |
Test Suspension |
Procedure Control (Reference Item) |
1 |
0.006 |
0.156 |
2 |
0.005 |
0.159 |
3 |
0.005 |
0.395 |
4 |
0.024 |
0.669 |
5 |
0.03 |
0.672 |
6 |
0.03 |
0.712 |
7 |
0.033 |
0.7333 |
8 |
0.033 |
0.78 |
9 |
- |
- |
10 |
- |
- |
11 |
0.038 |
0.79 |
12 |
0.036 |
0.808 |
13 |
0.036 |
0.822 |
14 |
- |
- |
15 |
0.035 |
0.834 |
16 |
0.038 |
0.839 |
17 |
- |
- |
18 |
0.038 |
0.849 |
19 |
0.036 |
0.847 |
20 |
0.039 |
0.859 |
21 |
0.038 |
0.859 |
22 |
0.039 |
0.869 |
23 |
0.042 |
0.869 |
24 |
0.041 |
0.876 |
25 |
- |
- |
26 |
0.042 |
0.879 |
27 |
0.038 |
0.863 |
28 |
0.038 |
0.853 |
TABLE 3: PERCENT BIODEGRDATION RESULTS
No. of Days |
Test Suspension |
Procedure Control (Reference Item) |
1 |
0.44 |
9.07 |
2 |
0.36 |
6.55 |
3 |
0.36 |
1.80 |
4 |
1.75 |
9.55 |
5 |
2.18 |
27.75 |
6 |
2.18 |
44.56 |
7 |
2.40 |
49.13 |
8 |
2.40 |
56.46 |
9 |
- |
- |
10 |
- |
- |
11 |
2.77 |
60.18 |
12 |
2.62 |
64.14 |
13 |
2.62 |
69.49 |
14 |
- |
- |
15 |
2.55 |
70.51 |
16 |
2.77 |
71.71 |
17 |
- |
- |
18 |
2.77 |
73.81 |
19 |
2.62 |
74.89 |
20 |
2.84 |
76.22 |
21 |
2.77 |
76.52 |
22 |
2.84 |
76.82 |
23 |
3.06 |
76.52 |
24 |
3.02 |
77.24 |
25 |
- |
- |
26 |
3.06 |
77.72 |
27 |
2.80 |
77.96 |
28 |
2.80 |
77.36 |
Table 4: BOD28, THOD AND % BIODEGRADATION VALUES
Method details |
BOD28(mgO2/mg) |
ThOD (mgO2/mg) |
% Biodegradation |
Test Item |
0.0385 mgO2/mg |
1.373 mgO2/mg |
2.80 % |
Reference Item |
1.288 mgO2/mg |
1.665 mgO2/mg |
77.36 % |
TABLE 5: pH OF RESPIROMETER FLASK
Respirometer Flask |
pH on 0th Day |
pH on 28th Day |
Inoculum Blank(Control) B1 |
7.4 |
7.7 |
B2 |
7.4 |
7.7 |
Test Suspension B1 |
7.4 |
7.8 |
B2 |
7.4 |
7.7 |
Procedure Control B1 |
7.5 |
7.7 |
B2 |
7.5 |
7.7 |
Description of key information
The test item disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (CAS No. 4553-89-3) undergoes 2.80 % biodegradation after 28 days in the test condition. Thus, the test item can be inferred/considered as not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Data available for target Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate for the endpoint Biodegradation in water are summarized as follows:
In key study, 28-days Manometric respirometry test following the OECD guideline 301F was conducted to determine the ready biodegradability of disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate(CAS No. 4553-89-3). The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of disodium-4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (CAS No. 4553-89-3) was observed to be 0.0385 mgO2/mg. ThOD was calculatedas 1.373 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was found to be 2.80%. Based on the results, the test item, under the test conditions, was found to be not readily biodegradable at 20 ± 1°C over a period of 28 days.
In supporting study, Estimation Programs Interface Suite (EPI suite, 2017) was run to predict the biodegradation potential of the test compound Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate), CAS No. 4553-89-3, in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that the test chemical Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) is not expected to be readily biodegradable.
Thus based on available data for target it is concluded that Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) is not expected to be readily biodegradable in water.
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