1H-Benz[e]indolium, 1,1,2,3-tetramethyl-, 4-methylbenzenesulfonate (1:1)

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Test accoding to GLP and the study procedures described in this report were based on the most recent OECD and EC guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU method B.47 (Bovine corneal opacity and permeability test method for identifying ocular corrosives and severe irritants)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,1,2,3-tetramethyl-1H-benzo[e]indol-3-ium 4-methylbenzene-1-sulfonate

Cas Number:

141914-99-0

Molecular formula:

C23H25NO3S

IUPAC Name:

1,1,2,3-tetramethyl-1H-benzo[e]indol-3-ium 4-methylbenzene-1-sulfonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Identification: V173520
Appearance: White powder (determined by WIL Research Europe)
Batch: AN
Purity/Composition: Not indicated
Test substance storage: At room temperature
Stable under storage conditions until: 20 April 2017 (expiry date)

Purity/composition correction factor: No correction factor required
Test substance handling: No specific handling conditions required
Chemical name (IUPAC), synonym or trade name: 1,1,2,2-tetramethyl-1H-Benz(e)indolium 4- methylbenzenesulfonate
CAS Number: 141914-99-0
Molecular formula: C23H25NO3S
Molecular weight: 395.53
pH (1% in water, indicative range) : 4.1 – 4.03 (determined by WIL Research Europe B.V. )
Solubility in vehicle: Physiological saline: Not indicated
Stability in vehicle: Physiological saline: Not indicated

Test animals / tissue source

Species:

other: Bovine eyes

Details on test animals or tissues and environmental conditions:

Bovine eyes from young cattle were obtained from the slaughterhouse (Vitelco, - 's Hertogenbosch, The Netherlands), where the eyes were excised by a slaughterhouse employee as soon as possible after slaughter.
Eyes were collected and transported in physiological saline in a suitable container under cooled conditions.

Test system

Vehicle:

physiological saline

Remarks:

A 20% (w/v) solution of V173520 was prepared in physiological saline (Eurovet Animal Health, Bladel, The Netherlands).

Controls:

yes

Amount / concentration applied:

V173520 was tested in a 20% (w/v) solution.

Duration of treatment / exposure:

240 min

Details on study design:

Treatment of corneas and opacity measurements

750 μl of either the negative control, positive control (20% (w/v) Imidazole solution) or 20% (w/v) test substance was introduced onto the epithelium of the cornea. The holder was slightly rotated, with the corneas maintained in a horizontal position, to ensure uniform distribution of the solutions over the entire cornea. Corneas were incubated in a horizontal position for 240 ± 10 minutes at 32 ± 1°C. After the incubation the solutions were removed and the epithelium was washed at least three times with MEM with phenol red (Eagle’s Minimum Essential Medium Life Technologies). Possible pH effects of the test substance on the corneas were recorded. Each cornea was inspected visually for dissimilar opacity patterns. The medium in the posterior compartment was removed and both compartments were refilled with fresh cMEM and the opacity determinations were performed.

Results and discussion

In vivo

Results

Irritation parameter:

other: In vitro irritancy scores

Basis:

other: range

Time point:

other: after 240 minutes

Score:

394 - 415

Max. score:

415

Irritant / corrosive response data:

The corneas treated with V173520 showed opacity values ranging from 383 to 385 and permeability values ranging from 0.720 to 2.018. The corneas were black after the 240 minutes of treatment with V173520. No pH effect of the test substance was observed on the rinsing medium. Hence, the in vitro irritancy scores ranged from 394 to 415 after 240 minutes of treatment with V173520.

Applicant's summary and conclusion

Interpretation of results:

Category 1 (irreversible effects on the eye) based on GHS criteria

Remarks:

Migrated information

Conclusions:

Since V173520 induced an IVIS ≥ 55, it is concluded that V173520 induces serious eye damage in the Bovine Corneal Opacity and Permeability test under the experimental conditions described in this report and should be classified category 1 according to the Globally Harmonized System of Classification and Labeling of Chemicals (GHS) of the United Nations.