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EC number: 210-568-3 | CAS number: 618-88-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Data is from study report
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- Assesment of a possible irritating potential to the eye and to the eye mucosa
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5-nitroisophthalic acid
- EC Number:
- 210-568-3
- EC Name:
- 5-nitroisophthalic acid
- Cas Number:
- 618-88-2
- Molecular formula:
- C8H5NO6
- IUPAC Name:
- 5-nitroisophthalic acid
- Test material form:
- solid
- Details on test material:
- IUPAC name: 5-Nitrobenzene-1,3-dicarboxylic acid
Mol. formula: C8H5NO6
Molecular Weight: 211.128 gm/mol
Smiles: OC(=O)c1cc(cc(c1)[N+](=O)[O-])C(=O)O
InChI: InChI=1S/C8H5NO6/c10-7(11)4-1-5(8(12)13)3-6(2-4)9(14)15/h1-3H,(H,10,11)(H,12,13)
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Weight at study initiation:
Male: 2.66 kg
Female: 2.45 kg
- Housing: Cage made of stainless steel with wire mesh walk floors. Floor area: 40cm x 51 cm
- Diet (e.g. ad libitum): KLIBA 341. 4 MM; FIRMA KLINGENTALMUEHLE AG CH-4303 KAISERAUGST. SWITZERLAND (ABOUT 130 G PER ANIMAL PER DAY)
- Water : About 250ml tap water per animal per cage
- Acclimation period: Atleast 8 days before the beginning of the study: Same housing conditions as during the study.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-24°C
- Humidity (%): 30-70%
- Air changes (per hr): Fully air conditioned
- Photoperiod (hrs dark / hrs light): 12h/12h (6.00-18.00 hours/18.00-6.00 hours)
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 0.1 ml bulk volume ( About 43 mg of the comminuted test substance)
- Duration of treatment / exposure:
- 1Hr; 24Hrs; 48Hrs; 72Hrs; 8D after application.
- Observation period (in vivo):
- 8 days
- Number of animals or in vitro replicates:
- 1 male
2 females - Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): Not specified
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 72 h
- Score:
- > 3 - < 3.7
- Max. score:
- 3.2
- Reversibility:
- not specified
- Remarks on result:
- other: Severe eye irritation
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 72 h
- Score:
- >= 0 - <= 0.3
- Max. score:
- 0.1
- Reversibility:
- not specified
- Remarks on result:
- other: Normal
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 72 h
- Score:
- >= 1.3 - <= 2
- Max. score:
- 1.7
- Reversibility:
- not specified
- Remarks on result:
- other: Well defined
Any other information on results incl. tables
Table 1: Body weight data
Animal |
1 |
2 |
3 |
Animal no. |
0201 |
0186 |
0161 |
Body weight (kg) |
2.50 |
2.40 |
2.66 |
Sex |
Female |
Female |
Male |
Table 2: Scores of eye irritation study
Readings |
Animal |
Cornea OP |
Iris |
Conjunctiva |
Symptoms |
|
Red |
SW |
|||||
1 hour |
1 |
2 |
0 |
2 |
2 |
Pupil contracted |
|
2 |
3 |
0 |
2 |
2 |
Pupil contracted |
|
3 |
2 |
0 |
2 |
2 |
|
24 hour |
1 |
3 |
0 |
2 |
2 |
S3 |
|
2 |
3 |
0 |
2 |
2 |
S/RE/S3 |
|
3 |
3 |
0 |
2 |
2 |
S3/S |
48 hour |
1 |
3 |
0 |
2 |
1 |
S3/S |
|
2 |
3 |
0 |
2 |
2 |
S/RE/S3 |
|
3 |
4 |
0 |
2 |
2 |
S3/S |
72 hour |
1 |
3 |
1 |
2 |
1 |
S3/S |
|
2 |
3 |
0 |
2 |
1 |
S/RE/S3 |
|
3 |
4 |
0 |
2 |
2 |
S3/S |
8 day |
1 |
3 |
1 |
2 |
0 |
S/RE/S3/PC/S4 |
|
2 |
4 |
0 |
2 |
1 |
S/RE/S3/LH/S4 |
|
3 |
4 |
1 |
2 |
2 |
S3/S/MV/S4 |
Mean |
1 |
3.0 |
0.3 |
2.0 |
1.3 |
|
|
2 |
3.0 |
0.0 |
2.0 |
1.7 |
|
|
3 |
3.7 |
0.0 |
2.0 |
2.0 |
|
Mean |
|
3.2 |
0.1 |
2.0 |
1.7 |
|
Explanation of symptoms:
S3: Loss of corneal tissue
S4: Study disconnected after 8 day because of severe opacity
LH: Loss of hair at margins of eyelids
MV: Marginal vascularization of the cornea
PC: Pupil contraction
RE: Small retraction in the eyelids
S: Suppuration
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- The test substance was observed to be irritating to the Eye when eye irritation study was conducted on rabbits.
- Executive summary:
Study, according to OECD guideline 405, was conducted on rabbit (strain Vienna White) to observe the eye irritation potential of test substance. 0.1 ml bulk volume (about 43 mg of comminuted test substance) was applied to the conjunctival sac of the right eyelid of the test animals. One male and two female animals were tested. Readings were taken at 1H, 24H, 48H, 72H, 8 Day after the application.Clinical symptoms were observed within one hour of the application and at the end of observations period (8 days), symptoms like loss of corneal tissue, loss of hair at the margins of eyelid, marginal vascularization of the cornea, small retractions in the eyelid were observed. Based on the symptoms, the following scores for ocular lesions were assigned: cornea score -3.2, iris score -0.1 and conjunctivae score 1.7. Accordingly the substance is classified as irritating to eyes. .
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