2,4,6-trichloro-1,3,5-triazine

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: No GLP compliance

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

Only four strains were tested (OECD 471 five)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: 84/449/EEC.

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2,4,6-trichloro-1,3,5-triazine

EC Number:

203-614-9

EC Name:

2,4,6-trichloro-1,3,5-triazine

Cas Number:

108-77-0

Molecular formula:

C3Cl3N3

IUPAC Name:

2,4,6-trichloro-1,3,5-triazine

Details on test material:

Cyanuric chloride, purity >95%.

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA97a, TA98, TA100 and TA102

Metabolic activation:

with and without

Metabolic activation system:

Male rat liver S9 mix (Aroclor 1254 induced)

Test concentrations with justification for top dose:

1, 10, 100 and 500 μg/plate (based on toxicity in TA97a).

Vehicle / solvent:

DMSO

Controls

Controls 1

Untreated negative controls:

yes

Remarks:

vehicle (DMSO)

Positive controls:

yes

Positive control substance:

other: 4-nitro-o-phenylenediamine

Remarks:

(TA98) (Without activation)

Controls 2

Untreated negative controls:

yes

Remarks:

vehicle (DMSO)

Positive controls:

yes

Positive control substance:

sodium azide

Remarks:

(TA100) (Without activation)

Controls 3

Untreated negative controls:

yes

Remarks:

vehicle (DMSO)

Positive controls:

yes

Positive control substance:

4-nitroquinoline-N-oxide

Remarks:

(TA97a, TA102) (Without activation)

Controls 4

Untreated negative controls:

yes

Remarks:

vehicle (DMSO)

Positive controls:

yes

Positive control substance:

other: 2-aminofluorene

Remarks:

(TA97a, TA100, TA102) (With activation)

Controls 5

Untreated negative controls:

yes

Remarks:

vehicle (DMSO)

Positive controls:

yes

Positive control substance:

benzo(a)pyrene

Remarks:

(TA98) (With activation)

Statistics:

Statistic method not performed.

Results and discussion

Any other information on results incl. tables

Tester strain	Test result(A)
	Without activation	With activation
TA97a	-	-
TA98	-	-
TA100	-	-
TA102	-	-

(A) +/- : positive/negative result; positive controls gave expected responses.

Applicant's summary and conclusion

Conclusions:

Not mutagenic.

Executive summary:

The mutagenic activity of cyanuric chloride was tested according to the Ames test, in bacterial strainsTA97a, TA98, TA100 and TA102, at a concentration of 1, 10, 100 and 500μg/plate. The procedure followed was plate incorporation assay, according to OECD 471. Cyanuric chloride was found to be not mutagenic.