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EC number: 232-094-6 | CAS number: 7786-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 2010 till May 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- One female animal was slightly below 200 g but this animal was only of 2 g below the accepted range, it was included in the study.
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Deviations:
- yes
- Remarks:
- One female animal was slightly below 200 g but this animal was only of 2 g below the accepted range, it was included in the study.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- magnesium chloride hexahydrate
- IUPAC Name:
- magnesium chloride hexahydrate
- Reference substance name:
- 7791-18-6
- Cas Number:
- 7791-18-6
- IUPAC Name:
- 7791-18-6
- Details on test material:
- water content (specification): 51-55 % (53.4 %)
Colour: colourless
Physical state: solid, crystals
Storage: at room temperature, in a tightly closed package
Solvent: water
Stability after opening: instable after repeated contact to air
pH: 5.5 - 7.0 (5 % solution at 20 °C)
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Magnesium chloride hexahydrate
- EC-Number: 232-094-6
- Physical state: Colourless; Solid, crystals
- Stability after opening: Instable after repeated contact to air
- Storage condition of test material: At room temperature, in a tightly closed package.
- pH: 5.5 - 7.0 (5% solution at 20 °C)
- Solvent: Water
No further information on the test material was stated.
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Species/strain: Healthy rats, WISTAR Crl: WI(Han) (Full-Barrier)
Source: Charles River, 97633 Sulzfeld, Germany
The female animals were nulliparous and non-pregnant.
Body weight at the beginning of the study: females: 198 - 222 g, males: 241 – 262 g
Age at the beginning of the study: females: 18 - 19 weeks old, males: 8 -9 weeks old
The animals were derived from a controlled full-barrier maintained breeding system (SPF).
Housing and Feeding Conditions: full barrier in an air-conditioned room, temperature: 22 (+/-3) °C, relative humidity: 55 (+/- 10)%, artificial light, sequence being 12 hours light, 12 hours dark, air change: 10 x / hour, free access to Altromin 1324 maintenance diet for rats and mice, free access to tap water, sulphur acidified to a pH value of approx. 2.8 (drinking water, municipal residue control, microbiological control at regular
intervals), the animals were kept individually in IVC cages, type III H, polysulphone cages on Altromin saw fibre bedding, adequate acclimatisation period.
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- water
- Remarks:
- Aqua ad injectionem
- Details on dermal exposure:
- Approximately 24 hours before the test, the fur was removed from the dorsal area of the trunk by using an electric clipper. Care was taken to avoid abrading the skin, and only animals with healthy intact skin were used.
No less than 10 % of the body surface was cleared for the application.
The test item was applied at a single dose, uniformly over an area which was approx. 10 % of the total body surface. - Duration of exposure:
- The test item was held in contact with the skin throughout a 24-hour period. At the end of the exposure period residual test item was not removed.
- Doses:
- 2000 mg/kg body weight
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- Prior to the application, a detailed clinical observation was made of all animals.
All animals were observed for 14 days after dosing.
The animals were weighed on day 1 (prior to the application) and on days 8 and 15.
A careful clinical examination was made several times on the day of dosing (at least once during the first 30 minutes and with special attention given during the first 4 hours post-dose). As soon as symptoms were noticed they were recorded. Thereafter, the animals were observed for clinical signs once daily until the end of the observation period. All abnormalities were recorded.
Cageside observations included changes in the skin and fur, eyes and mucous membranes. Also respiratory, circulatory, autonomic and central nervous systems and somatomotor activity and behaviour pattern were examined. Attention was directed to observations of tremors, convulsions, salivation, diarrhea, lethargy, sleep and coma.
At the end of the observation period, the animals were sacrificed by an overdosage of pentobarbital injected intraperitoneally. All animals were subjected to gross necropsy. All gross pathological changes were recorded. - Statistics:
- Signs of erythema and oedema were assessed using the scoring system laid down in OECD Guideline 404.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No
- Clinical signs:
- other: The test item showed slight signs of dermal irritation (slight scratches) after a single dose application for one female (n°14) on day 3 and 4. The clinical signs observed were seen only on the day of application and may partly be due to the stress induc
- Gross pathology:
- At necropsy, female no. 14 showed changes of the tissue on the large intestine, which was filled with liquid. As this finding was seen in only one animal and was not associated with any specific clinical signs, this finding was most probably not test item related.
- Other findings:
- No
Any other information on results incl. tables
Absolute Body Weights in g and Body Weight Gain in %
Animal No. / Sex |
Day 1 | Day 8 | Day 15 | % Day 1 - 15 |
11 female | 222 | 219 | 219 | -1 |
12 female | 201 | 208 | 213 | 6 |
13 female | 218 | 216 | 215 | -1 |
14 female | 214 | 217 | 215 | 0.5 |
15 female | 198 | 195 | 200 | 1 |
21 male | 262 | 288 | 315 | 20 |
22 male | 256 | 275 | 303 | 18 |
23 male | 241 | 268 | 291 | 21 |
24 male | 244 | 264 | 286 | 17 |
25 male | 247 | 273 | 302 | 22 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the conditions of this study, the dermal LD50 was determined to be > 2000 mg Magnesium chloride hexahydrate/kg body weight.
- Executive summary:
This study was a limit test of the acute dermal method (OECD 402) in male and female Wistar rats carried out according to the OECD guideline 402.
Under the conditions of the present study, single dermal application of the test item Magnesium chloride hexahydrate to rats at a dose of 2000 mg/kg body weight was associated with slight signs of toxicity and irritation, but no mortality.
The dermal LD50 was determined to be > 2000 mg Magnesium chloride hexahydrate/kg body weight.
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