Lanthanum chloride, anhydrous

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication/study report which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Lanthanum nitrate 6*H2O
- Molecular formula (if other than submission substance): La(NO3)3 * 6H2O
- CAS No.: 10277-43-7
- Analytical purity: > 99%

Method

Target gene:

his operon

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA100, TA1535, TA97, TA98

Metabolic activation:

with and without

Metabolic activation system:

Liver S-9 Fraction of Aroclor 1254-induced male Sprague-Dawley rat and Sprague-Dawley hamster. 10 and 30% S-9 mixes were tested.

Test concentrations with justification for top dose:

TA100/TA1535:
without and with activation (10% HLI, 10% RLI): 0, 3.3, 10, 33, 100 and 333 µg/plate;
with activation (30% HLI, 30% RLI): 0, 33, 100, 333, 1000 and 3333 µg/plate

TA97/TA98:
without activation and with activation (10% RLI): 0, 100, 333, 1000, 3333 and 10000 µg/plate
with activation (10% HLI, 30%HLI, 30% RLI): 0, 33, 100, 333, 1000 and 3333 µg/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: water

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: without metabolic activation: sodium azide (TA1535 and TAlOO), 9-aminoacridine (TA97), 4-nitro-o-phenylenediamine (TA98); with metabolic activation: 2-aminoanthracene for all strains.

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period: 20 min.
- Exposure duration: 2 days

NUMBER OF REPLICATIONS: 3

DETERMINATION OF CYTOTOXICITY
- Method: relative total growth

Evaluation criteria:

The colonies were machine counted unless precipitate was present. If precipitate were present, the colonies were counted visually. Mean values and standard deviations were calculated (three replicates).
A substance was judged mutagenic (+) or weakly mutagenic (+W) if it produced a reproducible, dose-related response over the solvent control, under a single metabolic activation condition, in replicate trials. A chemical was judged questionable (?) if the results of individual trials were not reproducible, if increases in his+ revertants did not meet the criteria for a “+W” response, or if only single doses produced increases in his+ revertants in repeat trials. It was not necessary for a response to reach two-fold over background for a trial to be judged mutagenic.
Chemicals were judged nonmutagenic (-) if they did not meet the criteria for a mutagenic or questionable response.

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA100, TA 1535, TA 97, TA98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

only observed with TA97 at 333 µg/plate and higher concentrations

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Mean number (with standard deviation, SEM) of revertants/plate with each bacterial strain:

(NA = without activation, HLI = liver S-9 fraction from hamster, RLI = liver S-9 frcation from rat, p = precipitate present in plates, x = precipitate present with toxicity, s = slight clearing of background lawn)

TA100
	NA		10% HLI		30% HLI		10% RLI		30% RLI
µg/plate	mean	sem	mean	sem	mean	sem	mean	sem	mean	sem
0	94	4.7	124	3.4	137	8.5	105	7.4	132	4.7
3.3	94	8.0	120	2.0			122	9.1
10	93p	1.7	111p	13.5			132p	1.5
33	102p	7.5	116p	8.5	147	7.5	123p	11.8	143	7.2
100	101p	6.4	107p	3.5	116	1.5	121p	5.2	141p	3.3
333	97p	0.9	113p	4.4	143	7.6	112p	10.1	149p	2.2
1000					130p	1.0			132p	5.0
3333					117p	7.3			106p	5.8
pos	746	19.2	302	13.5	679	12.3	755	18.2	1416	29.8

TA1535
	NA		10% HLI		30% HLI		10% RLI		30% RLI
µg/plate	mean	sem	mean	sem	mean	sem	mean	sem	mean	sem
0	11	2.1	11	1.5	10	1.9	10	1.8	10	0.3
3.3	6	1.5	13	3.4			12	2.1
10	11p	2.7	13p	1.5			12p	3.8
33	7p	2.2	12p	1.2	13	1.7	14p	0.7	10	2.4
100	9p	0.9	10p	1.0	13	2.6	10p	2.1	10	0.3
333	10p	0.7	11p	0.6	9p	1.8	10p	0.6	13p	1.5
1000					12p	2.1			8p	0.7
3333					10p	0.9			8p	0.9
pos	385	17.3	39	4.8	96	10.7	184	6.2	156	15.5

TA97
	NA		10% HLI		30% HLI		10% RLI		30% RLI
µg/plate	mean	sem	mean	sem	mean	sem	mean	sem	mean	sem
0	127	8.7	114	11.7	156	13.0	115	3.2	199	2.8
33			128p	5.3	161	4.8			184	3.7
100	127p	1.0	123p	9.9	159	6.7	79p	4.6	163p	12.2
333	131p	2.0	105p	10.1	155p	3.8	92p	4.6	129p	3.8
1000	119p	7.1	94p	7.0	122p	5.2	109p	0.9	118p	1.0
3333	99p	4.3	86x	3.9	99p	6.4	80p	6.6	118p	9.4
10000	86x	2.0					60p	7.5
pos	260	19.5	1078	29.4	1088	24.1	2819	19.2	1016	13.2

TA98
	NA		10% HLI		30% HLI		10% RLI		30% RLI
µg/plate	mean	sem	mean	sem	mean	sem	mean	sem	mean	sem
0	41	5.9	31	3.5	37	1.5	29	5.8	32	1.5
33			35	1.9	27	4.5			32	3.5
100	31p	4.9	38	4.9	27	1.0	32	1.7	30	0.3
333	37p	4.4	34	4.1	26p	1.7	33	4.7	36	1.2
1000	48p	2.3	28p	4.0	26p	3.5	32	4.1	32	3.0
3333	45s	5.6	27p	3.8	26p	4.1	25p	0.3	29p	2.3
10000	37s	2.8					30p	3.6
pos	313	30.7	201	22.0	149	0.0	350	24.6	486	17.3