Molybdenum nickel tetraoxide

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23 Jun 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: ICCVAM recommended HET-CAM test method protocol (Nov. 2006)

Principles of method if other than guideline:

The in vitro study was performed to evaluate the irritating potential of molybdenum nickel tetraoxide by detection of damages in blood vessels under the chorioallantoic membrane of 9 day incubated chicken eggs.

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Kaiser-Friedrich-Straße 7, 55116 Mainz, Germany

Test material

Test material information

Constituent 1

Reference substance name:

Molybdenum nickel tetraoxide

EC Number:

238-034-5

EC Name:

Molybdenum nickel tetraoxide

Cas Number:

14177-55-0

Molecular formula:

MoO3*nNiO (with n in a range of 0.6 - 1)

IUPAC Name:

Reaction mass of molybdenum oxide and nickel oxide

Constituent 2

Reference substance name:

238-034-0

IUPAC Name:

238-034-0

Details on test material:

- Name of test material (as cited in study report): Molybdenum nickel tetraoxide
- Molecular formula: NiMoO4
- Physical state /appearance: light yellow powder
- Analytical purity: >99%
- Lot/batch No.: GRA20100315
- Expiration date of the lot/batch: Feb 2012
- Storage condition: room temperature 20 +/- 5°C; closed vessel

Test animals / tissue source

Species:

other: Lohmann selected Leghorn chicken's eggs

Strain:

other: Lohmann selected Leghorn chicken's eggs

Details on test animals or tissues and environmental conditions:

The eggs were obtained from LSL Rhein Main Geflügelvermehrungsbetrieb, Dieburg, Germany.

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: not applicable

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 300 µL (mean weight: 0.1429 g)

Duration of treatment / exposure:

not applicable; see "any other information on materials and methods"

Observation period (in vivo):

not applicable; see "any other information on materials and methods"

Number of animals or in vitro replicates:

not applicable; see "any other information on materials and methods" 15 eggs (3 for negative control, 6 for 2 different positive controls, 6 for the test item)

Results and discussion

Any other information on results incl. tables

Table 1: Observations and calculated Irritation Score - Evaluation of Molybdenum nickel tetraoxide in the HET-CAM Test

Test Group	Amount applied	Time until Haemorrhage	Time until Lysis	Time until Coagulation	Mean Irritation Score
		seconds	seconds	seconds
Negative Control	300 µL	301	301	301	0.00
	300 µL	301	301	301
	300 µL	301	301	301
Positive Control 0.1-N NaOH	300 µL	9	22	15	20.02
	300 µL	7	20	13
	300 µL	7	21	15
Positive Control 1% SDS	300 µL	14	26	301	11.15
	300 µL	16	29	301
	300 µL	16	27	301
Molybdenum nickel tetraoxide	0.1659 g	301	301	301	0.00
	0.1346 g	301	301	301
	0.1364 g	301	301	301
	0.1345 g	301	301	301
	0.1418 g	301	301	301
	0.1441 g	301	301	301

In the negative control, no signs of irritation were observed.

Both positive controls showed severe irritation.

The test item Molybdenum nickel tetraoxide showed no irritation.

In conclusion, it can be stated that in this study and under the experimental conditions reported, the test item Molybdenum nickel tetraoxide possesses no irritation potential.

Table 2: Classification of the irritation potential

Irritation Score (IS)	Classification
0 - 0.9	not irritant
1 - 4.9	slightly irritant
5 - 8.9	moderately irritant
9 - 21	severely irritant

Table 3: Validity criteria and findings

Parameter	Criterion	Found	Assessment
IS of negative controls	< 0.9	0.0	ok
IS of positive controls	> 9	20.02 (NaOH) 11.15 (SDS)	ok

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information