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EC number: 413-550-5 | CAS number: 142068-96-0 H112339
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 1992 to February 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to recent EU test guidance in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Version / remarks:
- Directive 84/449/EEC
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- EC Number:
- 413-550-5
- EC Name:
- Octasodium 2-(8-(4-chloro-6-(3-((4-chloro-6-(3,6-disulfonato-2-(1,5-disulfonatonaphthalen-2-ylazo)-1-hydroxynaphthalen-8-ylamino)-1,3,5-triazin-2-yl)aminomethyl)phenylamino)-1,3,5-triazin-2-ylamino)-3,6-disulfonato-1-hydroxynaphthalen-2-ylazo)naphthalene-1,5-disulfonate
- Cas Number:
- 142068-96-0
- Molecular formula:
- Hill formula: C53H28Cl2N14Na8O26S8
- IUPAC Name:
- octasodium 5-[(4-chloro-6-{[4-({[4-chloro-6-({7-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-8-hydroxy-3,6-disulfonatonaphthalen-1-yl}amino)-1,3,5-triazin-2-yl]amino}methyl)phenyl]amino}-1,3,5-triazin-2-yl)amino]-3-[2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate
- Details on test material:
- - Name of test material (as cited in study report): H112339
- Physical state: Powder
- Analytical purity: 71.6%
- Lot/batch No.: NBY 405/74
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Radiolabelling:
- no
Study design
- Analytical monitoring:
- yes
- Details on sampling:
- Not specified.
- Buffers:
- pH 4.0 Buffer Solution
4ml of 0.1N sodium hydroxide solution added to 500ml of 0.1M potassium biphthalate and diluted to 1 litre with distilled water.
pH 7.0 Buffer Solution
296ml of 0.1N sodium hydroxide solution added to 500ml of 0.1M monopotassium phosphate and diluted to 1 litre with distilled water.
pH 9.0 Buffer Solution
213ml of 0.1N sodium hydroxide solution added to 500ml of Q.1M boric acid in 0.1M potassium chloride and diluted to 1 litre with distilled water.
All buffer solutions were prepared in distilled water which had been degassed with helium. The pH of each solution was measured and adjusted as required with the appropriate reagent as detailed above. - Estimation method (if used):
- Not applicable.
- Details on test conditions:
- Not specified.
Duration of testopen allclose all
- Duration:
- 7 d
- pH:
- 4
- Temp.:
- 50
- Initial conc. measured:
- 1.066 g/L
- Duration:
- 7 d
- pH:
- 7
- Temp.:
- 50
- Initial conc. measured:
- 1.03 g/L
- Duration:
- 7 d
- pH:
- 9
- Temp.:
- 50
- Initial conc. measured:
- 1.09 g/L
- Number of replicates:
- 2
- Positive controls:
- no
- Negative controls:
- no
Results and discussion
- Preliminary study:
- Less than 10% hydrolysis was obtained at pH's 7.0 and 9,0 so no further testing was required. At pH 4.0 approximately 50% hydrolysis occurred and the linear plot obtained indicates that the reaction is pseudo-first order. Further testing at pH 4.0 was therefore carried out at 35°C to obtain an extrapolated value for the rate constant at 25°C.
- Test performance:
- None specified.
- Transformation products:
- not measured
- Details on hydrolysis and appearance of transformation product(s):
- Not measured
Dissipation DT50 of parent compoundopen allclose all
- pH:
- 4
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0.013 d-1
- DT50:
- 53.72 d
- Type:
- (pseudo-)first order (= half-life)
- Remarks on result:
- other: Calculated from data at 50deg C and 35 Deg C
- pH:
- 4
- Temp.:
- 35 °C
- Hydrolysis rate constant:
- 0.035 d-1
- DT50:
- 19.69 d
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 4
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.138 d-1
- DT50:
- 5 d
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 50 °C
- DT50:
- > 1 yr
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 50 °C
- DT50:
- > 1 yr
- Type:
- (pseudo-)first order (= half-life)
- Details on results:
- The hydrolysis of the test substance was studied at pH's 4, 7 and 9. Solutions of substance were prepared in the buffer solutions and these were stored in amber coloured flasks at 50°C. The concentration of the main component of substance was monitored over several days by high performance liquid chromatography (HPLC). Duplicate tests were carried out at each pH. Less than 10% hydrolysis was obtained at pH's7.0 and 9,0 so no further testing was required. At pH 4.0 approximately 50% hydrolysis occurred and the linear plot obtained indicates that the reaction is pseudo-first order. Further testing at pH 4.0 was therefore carried out at 35°C to obtain an extrapolated value for the rate constant at 25°C.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Result
kobs - 13.84 x 10-2 days-1 in pH 4 buffer at 50°C
t½ =5.01 days in pH 4 buffer at 50⁰C
kobs- 3.52 x 10-2 days-1 in PH 4 buffer at 35°C
t½ - 19.69 days in pH 4 buffer at 35°C
Extrapolated values
kobs - 1,29 x 10-2 days-1 in PH 4 buffer at 25°C
t½ - 53.72 days in pH 4 buffer at 25°C
Less than 10% hydrolysis was obtained at pH's 7.0 and 9,0 so no further testing was required - Executive summary:
The substance is considered to be stable to hydrolysis at neutral and basic pH’s. At acidic pH’s a moderate amount of degradation occurs.
Result
kobs- 13.84 x 10-2days-1in pH 4 buffer at 50°C
t½ =5.01 days in pH 4 buffer at 50⁰C
kOBS- 3.52 x 10-2days-1in pH 4 buffer at 35°C
t½ - 19.69 days in pH 4 buffer at 35°C
Extrapolated values
kobs- 1,29 x 10-2days-1in pH 4 buffer at 25°C
t½ - 53.72 days in pH 4 buffer at 25°C
The hydrolysis of the test substance was studied at pH's 4, 7 and 9. Solutions of Substance were prepared in the buffer solutions and these were stored in amber coloured flasks at 50°C. The concentration of the main component of Substance was monitored over several days by high performance liquid chromatography (HPLC). Duplicate tests were carried out at each pH. Less than 10% hydrolysis was obtained at pH's7.0 and 9,0 so no further testing was required. At pH 4.0 approximately 50% hydrolysis occurred and the linear plot obtained indicates that the reaction is pseudo-first order. Further testing at pH 4.0 was therefore carried out at 35°C to obtain an extrapolated value for the rate constant at 25°C.
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