m-terphenyl-2'-ol

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

m-terphenyl-2'-ol

EC Number:

219-401-9

EC Name:

m-terphenyl-2'-ol

Cas Number:

2432-11-3

Molecular formula:

C18H14O

IUPAC Name:

3-phenyl-[1,1'-biphenyl]-2-ol

Details on test material:

- Name of test material (as cited in study report): 2,6 Diphenylphenol
- Substance type: solid/beige
- Analytical purity: 100%
- Lot/batch No.: LV 83/2010

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was weighed in the required amounts for the test concentrations directly to the test vessel. Than 180 ml demineralized water were added. The mixtures were stirred for 20 h at 20.5-20.8 °C

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: activated sludge from the municipal wastewater treatment plant
- Preparation of inoculum for exposure: activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with tap water and the suspension was adjusted to 7.5 g/L dry matter
- Initial biomass concentration: 1.5 g/L dry matter per vessel

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

20.7 - 20.9 °C

pH:

7.8 - 7.9

Nominal and measured concentrations:

Nominal test concentrations: control, 62.4, 125, 250, 501, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: Erlenmeyer-flasks, 250 ml, 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per blank control (replicates): 2
- No. of vessels per reference substance (replicates): 1

EFFECT PARAMETERS MEASURED: respiration rate after 180 min

Reason for selection of test concentrations: According to the test guidelines, at least 5 concentrations in a geometric series with a separation factor of ≤ 3.2 should be used, preferably encompassing the range from 10 – 80% inhibition

Reference substance (positive control):

yes

Remarks:

3,5 dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

EC10

Effect conc.:

550 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: 95% CL: 474.8 - 630.5 mg/L

Effect concentrations 2

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

810 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: 795% CL: 46.7 - 872.6 mg/L

Effect concentrations 3

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 4

Duration:

180 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate