m-terphenyl-2'-ol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

m-terphenyl-2'-ol

EC Number:

219-401-9

EC Name:

m-terphenyl-2'-ol

Cas Number:

2432-11-3

Molecular formula:

C18H14O

IUPAC Name:

3-phenyl-[1,1'-biphenyl]-2-ol

Details on test material:

- Name of test material (as cited in study report): 2,6 Diphenylphenol
- Physical state: Solid / beige
- Analytical purity: 100%
- Lot/batch No.: LV 83/2010
- Expiration date of the lot/batch: 18 August 2012
- Stability under test conditions: stable
- Storage condition of test material: at room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Municipal activated sludge from the wastewater treatment plant of Mannheim, Germany.
- Storage conditions: The inoculum was collected on 17 September 2010 from the aeration tank of the plant and aerated in the laboratory until use. A suitable aliquot of the activated sludge suspension was sieved by a finely woven mesh with a mesh size about 1 mm. To reduce the content of inorganic carbon in the blank controls the activated sludge was aerated with carbon dioxide free air for about 72 hours at 22 ± 2° C.

- Preparation of inoculum for exposure: At the day of exposure the suspension was washed one time with tap water. Therefore the
aeration was stopped and the sludge was allowed to settle. After settling the supernatant was discarded and the remaining sludge suspension was filled up with tap water and the concentration oft the sludge was adjusted to 6.0 g/L dry weight.

- Concentration of sludge: Aliquots of 7.5 mL were added to the test vessels to obtain a activated sludge concentration
of 30 mg/L dry weight.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

23 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: according to guideline
- Solubilising agent: no
- Test temperature: 22 ± 2 °C
- pH: The pH-values in the test vessels were measured and adjusted to 7.4 ± 0.2 respectively, if necessary



TEST SYSTEM
- Culturing apparatus: 2 L incubation bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for two days before test start
- Measuring equipment: carbon dioxide trap
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: Usually twice a week the Total Inorganic Carbon
(TIC) values of the adsorption solutions of the first trap were determined and used for the
calculation of the produced carbon dioxide. After each sampling the second trap was moved
forward and the new trap with fresh sodium hydroxide solution was placed into the second
position. Each trap was analyzed separately.

SAMPLING
- Sampling frequency: Usually twice a week
- Sampling method: Samples for the TIC- and DOC-analysis were performed as repeat determination, using a TOC-analyzer equipped with an auto sampler (Shimadzu TOC-5000A). The system works with a combustion/non-disperse infrared gas analysis method.

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
- Other: Inhibition control

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

< 10

Sampling time:

28 d

Details on results:

2,6 Diphenylphenol was not readily biodegradable under the conditions of this test

BOD5 / COD results

Results with reference substance:

Degree of biodegradation of the reference substance after 14 days: 86 % CO2/ThCO2