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EC number: 220-395-5 | CAS number: 2752-17-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Following the read across strategy, this endpoint is covered by a key study performed with the structural analogue BDMAEE (N, N, N', N'-tetramethyl-2,2'-oxybis(ethylamine)). The justification for read across is attached in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 720 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- yes
- Conclusions:
- No reliable data is available with the test substance. Data generated with the analogue substance BDMAEE is used for ednpoint coverage. Under the conditions of this study, the 30 minute EC20 was >720 mg/L (nominal) based on respiration rate in industrial activated sludge. The same is assumed to be correct for the test substance.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Documentation of the older study falls short of today´s requirements, but corresponds to the standards at the time they were conducted; the quality of the study performance is nevertheless not influenced. Sufficient data suitable for assessment. Inoculum taken from industrial source.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- see below
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, industrial
- Details on inoculum:
- source: industrial waste water treatment plant of BASF AG, production site Ludwigshafen
final test concentration: 1 g/L dry weight - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 30 min
- Test temperature:
- room temperature: 20-25 °C
- pH:
- no details
- Nominal and measured concentrations:
- Dilution series from stock solutions (1000 mg/L nominal) resulted in the following concentrations: 720 and 360 mg/L (nominal).
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, Erlenmeyer-flask (250 ml nominal), liquid volume: 250 mL
- Aeration: sparged with air
- No. of vessels per concentration (replicates; test substance, TS): 1
- No. of vessels per control (replicates; blanc, BC): 0
- No. of vessels per concentration (replicates; positive control, reference substance, RS): 0
TEST MEDIUM / WATER PARAMETERS
- Culture medium: OECD test medium
OTHER TEST CONDITIONS
- Adjustment of pH: no pH adjustments during test
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
O2: on line measurement for approx. 10 min
pH: no data
TEST CONCENTRATIONS
- Spacing factor for test concentrations: <3.2
- Test item concentrations:720 and 360 mg/L (nominal)
Test conditions
Reference substance: not tested in parallel - Reference substance (positive control):
- no
- Key result
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 720 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Effect concentrations exceeding solubility of substance in test medium: no
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this study, the 30 minute EC20 was >720 mg/L (nominal) based on respiration rate in industrial activated sludge
Referenceopen allclose all
Reference value for blanc (mean value): 27 mg/L*h
Test concentration |
Respiration rate |
Respiration change |
[mg/L] |
[mg O2/L/h] |
[%] |
360 |
31 |
15 |
720 |
30 |
11 |
Respiration was stimulated by the test substance.
Description of key information
Following the read-across strategy, it is considered appropriate to cover this endpoint by a key study performed with the structural analogue BDMAEE (N, N, N', N'-tetramethyl-2,2'-oxybis(ethylamine)). A study performed under GLP conditions and according to OECD 209 showed a 30 min. EC20 >720 mg/L (nominal) based on respiration rate in industrial activated sludge.
Key value for chemical safety assessment
Additional information
No reliable data is available with the test substance. Data generated with the analogue substance BDMAEE is used for endpoint coverage. A study performed under GLP conditions and according to OECD 209 showed a 30 min. EC20 >720 mg/L (nominal) based on respiration rate in industrial activated sludge. The same is assumed to be correct for the test substance.
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