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EC number: 927-344-2
CAS number: -
Table 3- Percent inhibition
Loading rate (mg/l)
Based on the slope of the growth rates
Based on the areas under the growth curves
Table 4 - Cell concentrations per
This study was conducted to evaluate the
effects of the water-accommodated fractions (WAFs) of Hydrocarbons,
C11-C14, isoalkanes, cyclics, aromatics (2-25%) (MRD-04-991) on the
growth of the alga, Pseudokirchneriella subcapitata, in a 96-hour
static test, conducted according to OECD guideline 201.
Individual treatments were prepared by
adding the appropriate amount of test substance to 4.2 L of algal
nutrient media and sodium bicarbonate in glass aspirator bottles
(capacity 4.5 L) and stirring on magnetic stirplates using an
approximately 7% (of the static liquid depth) vortex for approximately
24 hours. After approximately one hour without stirring, the aqueous
portions (WAFs) were removed for testing. The actual loading rates were
0 mg/L (control), 0.26, 0.76, 1.7, 4.0, and 10 mg/L. The mean measured
concentrations were ND (Not Detected; control), 0.049, 0.16, 0.39, 0.97,
and 2.1 mg/L.
Twelve replicate chambers were established
for each treatment and the control. The test chambers were completely
filled (no headspace) with the appropriate WAF and were sealed with
ground glass stoppers. Each chamber contained two 14-mm glass spheres to
facilitate mixing. Test chambers were placed on two shaker tables and
oscillated at 100 rpm to keep the algae in suspension. The study was
performed under continuous light conditions (approximately 8346 Lux) at
approximately 24°C. The pH in the test solutions ranged from 7.5-7.6 at
the beginning of the test and from 8.1-9.8 at the end of the test. Three
replicates from each loading rate were sacrificed daily for cell density
Acute toxicity results are expressed as the
Effect Loading / Effect Concentration 50 (EL/EC50); that is, the loading
rate or concentration of test substance in dilution medium which is
calculated to result in a 50% reduction in growth derived from either
the average specific growth rate (r) or the area under the growth curves
(b) relative to the control for the specified time of exposure. The No
Observed Effect Loading Rate / No Observed Effect Concentration
(NOELR/NOEC) is the highest loading rate or concentration which does not
exhibit a statistical difference from the control.
The 72 and 96-hour ErL50 was 4.1 and 5.5
mg/L, respectively. The 72 and 96-hour EbL50 was 2.3 and 2.5 mg/L,
respectively. The 72 and 96-hour ErC50 was 0.94 and 1.2 mg/L,
respectively.The 72 and 96-hour EbC50 was 0.53 and 0.58 mg/L,
respectively. The 72 and 96-hour NOELR was 0.76 mg/L. The 72 and 96-hour
NOEC was 0.16 mg/L.
Growth of Raphidocelis
subcapitata at 72 hrs
Mean cell Concentration (million cells/ml)
Calculated mean area (A/ 1 million)
Mean reduction in A relative to controls (%)
Mean calculated µ
Mean reduction in µ relative to controls (%)
This study was done to determine the acute
toxicity of the test material to the alga Pseudokirchneriella
subcapitata. The algae were exposed to loading rates of 0, 0.1,
0.22, 0.46, 1.0, 2.2, 4.6, or 10 mg/l (WAF) of test substance for a
period of 72 hrs. There was no renewal of test solution. Cell counts
were taken at 0, 24, 48, and 72 hrs after start of exposure. The test
substance exhibited 72-hour EbL50 (biomass) and ErL50 (growth rate)
values of 4.6-10 ml/L with the alga, Pseudokirchneriella subcapitata. The
72-hr NOEL values for biomass and growth rate were 0.22 and 1 mg/L,
The acute toxicity of water
accomodated fractions (aqueous extrats) of Shellsol H has been
determined to the unicellular alga Raphidocelis subcapitata (formerly
The test media were prepared by adding
quantities of Shellsol H to culture medium appropriate to the test
organisms and stirring in sealed vessels for 49 hours). The strirring
period was demonstrated as being sufficient to ensure equilibration of
the shellsol H and aqueous phases. After stirring the contents of the
test vessels were left to settle for about 1 hour before drawing off the
WAF for use as the test media in the toxicity tests.
The concentrations of dissolved
hydrocarbons (derived from the shellsol H) in the test media was
determined during the toxicit tests usig gas chromatography. The
analyses showed that the concentration in the test media was <1%% of the
loading rate. The reduction in the concentration of dissolved
hydrocarbons in the test media during the test was ≤26%.
The acute toxicity to R. subcapitata
of water accomodated fractions of Shellsol H prepared at loading ranging
from 0.1 to 100 mg/l was determined in a 72h growth inhibition test
without renewal of the test media in sealed test vessels. The7h EL50
values (loading rates reducing the area under the growth curves and the
average specific growth rates by 50% after 72h exposure) were in the
range 10 -100 mg/l.
were exposed to water accomodated fractions (WAFs) of Hydrocarbons,
C9-C12, isoalkanes. cyclics, arornatics (2-25%) in two separate studies.
The 72-hr EL50 value for growth rate were found to be 4.1 and
4.6 -10 mg/l. The 72-hr NOELR values for growth rate were 0.76 and 0.22
EL50 for algal cultures exposed to WAFs of Hydrocarbons,
C10-C13, n-alkanes, isoalkanes, cyclics, aromatics (2-25%) were 10 -100
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