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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

One key 48-hour Daphnia magna immobilization test; a second supporting study was also available.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
68 mg/L

Additional information

The purpose of this study was to evaluate the acute toxicity of the reaction product of 2-

hydroxyethyl methacrylate (HEMA) with polyphosphoric acid (PPA), hereafter refered to as

HEMA Phosphate, to the freshwater cladoceran, Daphnia magna, over a 48-hour exposure

period. The definitive test was conducted with 4 replicate vessels per test level, with 5

daphnids each, exposed to nominal concentrations of 0 (water control), 6.3, 13, 25, 50, and

100 mg HEMA Phosphate/L. The mean measured concentrations were <LLQ (water

control), 6.79, 14.8, 28.3, 55.5, and 105 mg HEMA Phosphate/L. Because the mean measure

concentrations were within 20% of the target concentrations, the acute toxicity values for

HEMA Phosphate with Daphnia magna were determined based on nominal concentrations.

The results were as follows:

• The 24-hour EC50 = 75 mg/L (95% confidence limits not calculable)

• The 48-hour EC50 = 68 mg/L ( 95% confidence limits 64 – 73 mg/L)

• The 48-hour NOEC = 50 mg/L

An earlier study in Daphnia magna demonstrated a 48 -hr EC50 to be >100 mg/L.