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Biodegradation in water: screening tests

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Description of key information

The ready biodegradability of the reaction product of 2-hydroxyethyl methacrylate (HEMA) and
polyphoshoric acid (PPA) was determined using the OECD Guideline No. 301F: Manometric
Respirometry Test.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

The ready biodegradability of the reaction product of 2-hydroxyethyl methacrylate (HEMA) and

polyphoshoric acid (PPA) was determined using the OECD Guideline No. 301F: Manometric

Respirometry Test. Biodegradation of the reaction product of 2-hydroxyethyl methacrylate (HEMA)

and polyphoshoric acid (PPA) (55.7 mg/L equivalent to 54.6 mg/L as theoretical oxygen demand

[ThOD]) reached 93.1% based on biological oxygen demand (BOD) at the end of this 28-day test.

Biodegradation of the test material started at approximately 1.6 days after the addition of the test

material into the reaction mixture and exceeded 60% within 11.9 days of the initiation of the test,

which indicates that the 10-day window criteria described in the OECD 301F guideline was met.

Therefore, the reaction product of 2-hydroxyethyl methacrylate (HEMA) and polyphoshoric acid

(PPA) can be classified as “readily biodegradable.”

The results of this test met or exceeded OECD-specified criteria for viability of the inoculum,

pH, and control of temperature. Biodegradation of a reference material (aniline, 100 mg/L

equivalent to 241 mg/L as ThOD) exceeded 60% by 7.4 days, verifying the viability of the

activated sludge inoculum. A Toxicity Control mixture, containing both aniline (100 mg/L

equivalent to 241 mg/L as ThOD) and the test material (53.4 mg/L equivalent to 52.5 mg/L as

ThOD), showed no evidence for inhibition of the microbial inoculum by the test material.

Oxygen consumption and CO2 evolution observed in the reaction mixtures could be attributed

solely to biological activity, as no net O2 consumption or CO2 production was measured in an

Abiotic Control mixture containing the test material and a chemical sterilant (HgCl2, 248 mg/L).