3-methylpentane-1,5-diol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The study was well conducted by a GLP-compliant laboratory according to an OECD-guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-methylpentane-1,5-diol

EC Number:

224-709-1

EC Name:

3-methylpentane-1,5-diol

Cas Number:

4457-71-0

Molecular formula:

C6H14O2

IUPAC Name:

3-methylpentane-1,5-diol

Specific details on test material used for the study:

- Substance type: Colourless, clear liquid
- Physical state: liquid
- Analytical purity: 99.8%
- Impurities (identity and concentrations): 0.04% water; less than 0.02% CH3COOH.
- Lot/batch No.:WDK5014
- Stability under test conditions: stable (IR-tested)
- Storage condition of test material: stored in a refrigerator

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in dilution water at a concentration of 1,000 mg/L. From this stock test solution, the test solutions were prepared by further diluting with water (c= 100 mg/L).
- Controls: 1 control, dilution water.

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: orange killifish
- Source: Nakajima Pisciculture
- Length at study initiation (length definition, mean, range and SD): 2,0 cm (mean); 2.0-2.1 cm (n=10).
- Weight at study initiation (mean and range, SD): 0.14g (mean); 0.13 - 0.15 g (n=10).
- Feeding during test: no feeding


ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): same
- Type and amount of food: Commercially available Tetramin. No food was given 24h before the exposure.
- Health during acclimation (any mortality observed): only healthy looking fish were used for the eventual test.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

none

Test conditions

Hardness:

55.6

Test temperature:

23.8 - 24.5°C

pH:

7.0-7.6

Dissolved oxygen:

5.4-8.2 mg/L

Nominal and measured concentrations:

Nominal: 100 mg/L
Measured: Beginnig of exposure: 105 mg/L
End of exposure: 101 mg/L
mean: 103 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 3.0L glass vessels
- Renewal rate of test solution (frequency/flow rate): once after 48 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Kurume tap water
- Chlorine: lower than 0.02 mg/L


TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: A preliminary concluded that there was 0% mortality at a MPD concentration of 300 mg/L. Therefore the eventual test's MPS concentration was adjusted to 100 mg/L only. Dilution water was used as control.

Reference substance (positive control):

yes

Remarks:

Copper sulphate (II) pentahydrate; LC50 = 0.745 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

other: maximum 0% mortality concentration

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no abnormalities were observed in any of the test media.
- Mortality of control: none
- Other adverse effects control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no

Any other information on results incl. tables

Sublethal observations / clinical signs:

After 96 hours of exposure, none of the test or control animals showed any toxic symptoms.

None of the test or control animals were dead. Therefore the 96h LC50 for MPD was determined to be >100 mg/L.

Applicant's summary and conclusion

Conclusions:

The 96h LC50 for MPD against killifish was determined to be >100 mg/L.

Executive summary:

Acute toxicity of MPD against killifish was tested in a 96h- semi-static test (c MPD = 100 mg/L). Control fish were used.

After 96 hours, none of the test or control animals showed any signs of toxic symptoms or mortality.

The LC50 was determined to be >100 mg/L.