Reaction product of phthalogen-brilliantblue IF3G with phenylguanazole and iron(II)-chloride

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-06-12 (experimental phase)

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Version / remarks:

ISO-Vorschrift 8192-1986 (E)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron

EC Number:

256-526-8

EC Name:

[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron

Cas Number:

50293-39-5

Molecular formula:

C32H18FeN12

IUPAC Name:

[8,20-dihydro-8,20-diphenyl-5,24:12,17-diimino-7,10:22,19-dinitrilodibenz[f,p][1,2,4,9,11,12,14,19]octaazacycloicosinato(2-)-N25,N26,N27,N28]iron

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

- Name and location of sewage treatment plant where inoculum was collected: Laborkläranlage (OECD)
- Initial biomass concentration: 6 g/l dry matter

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Remarks on exposure duration:

not stated in report

Test conditions

Nominal and measured concentrations:

nominal 100, 1000, 1000 mg/l

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Key result

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks on result:

not determinable

Remarks:

no sufficient inhibition

Results with reference substance (positive control):

Test valid, positive control gave the appropriate results

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

positive control gave the appropriate result

Conclusions:

The study was conducted according to ISO-Vorschrift 8192-1986 (E) on the registered substance itself with minor deficiencies in documentation. However, the given data indicate that the study was well and scientifically reasonable performed. The positive control induced the appropriate response. Hence, the results can be considered as sufficiently reliable to assess the toxicity of the test item towards microorganisms. The determined EC50 was > 10000 mg/l, hence, the substance can be considered as non-toxic to activated sludge.

Executive summary:

The potential hazard of the test item towards microorganisms was tested in an ISO-Vorschrift 8192-1986 (E) study. Concentrations ranging from 100 to 10000 mg/l were applied to activated sludge and the respiration rate was determined. Concentrations up to 1000 mg/l did not result in an inhibition of the respiration rate, 10000 mg/l induced an inhibition of 25%. 3,5-dichlorophenol served as positive control and induced the appropriate results. The test item was non-toxic to activated sludge, the EC50 was determined as > 10000 mg/l.