Cuprate(4-), [μ-[[7,7'-[[6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diyl]diimino]bis[4-(hydroxy-κO)-3-[[2-(hydroxy-κO)-5-sulfophenyl]azo-κN1]-2-naphthalenesulfonato]](8-)]]di-, tetrasodium

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Vehicle:

yes

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Acclimation period: 20.06-15.07-1996
Acclimation conditions: temperature: 9.0-19.5˚C. Dissolved oxygen: > 80% air saturation value
At the end of the test a sample of 10 trout was weighed and measured. Mean length: 48.9 mm; mean weight: 1.10 g
The sizes of the animals were found to be in approximately normal Gaussian distribution.

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

286 mg CaCO3/L

Test temperature:

Range of temperature readings: 15.1-17.5˚C

pH:

Range of pH values: 8.0-8.8

Dissolved oxygen:

Range of dissolved oxygen values: 90-96% (air saturation value)

Reference substance (positive control):

yes

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

59 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 46-75

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

30 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 18-49

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 12-22

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

13 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 10-18

Sublethal observations / clinical signs:

Cumulative percent mortalities, test species: Oncorhynchus mykiss

	Concentration of test material (mg/l)					Control
Exposure period (hr)	10	18	32	56	100	0
24	0	0	0	28.6	100	0
48	0	57.1	57.1	57.1	100	0
72	14.6	100	85.7	100	100	0
96	42.9	100	85.7	100	100	0

Validity criteria fulfilled:

yes

Conclusions:

The estimated LC50 values of 59 mg/l at 24 hrs and 13 mg/l at 96 hrs support these observations.

Description of key information

LC50 values is 13 mg/l at 96 hrs.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

13 mg/L

Additional information

This report contains a study to investigate the acute toxicity of BENZANIL RUBINE 2BL (Direct Red 83-1) to rainbow trout (oncorhynchus mykiss). The objective of this study was to determine the 96 hr median lethal concentration (LC50). 5 concentrations were tested 10, 18, 32, 56 and 100 mg/l.

The results of this study indicate that at concentration above 18 mg/l is fairly rapidly lethal to rainbow trout. The estimated median period of survival, of the exposed fish, obtained for at levels of 56, 32 and 18 mg/l, were 39hr, 57hr and 47hr respectively. The lowest test concentration which caused 100% mortality of exposure fish within 96 was 18 mg/l. The highest no-observed lethal effect concentration of the test substance to rainbow trout could not be determined as there was 42.9% mortality at the lowest test concentration.

Dilution of the sample from 18 mg/l to 10 mg/l significantly increases the survival time of the trout. The estimated LC50 values of 59 mg/l at 24 hrs and 13 mg/l at 96 hrs support these observations.

A mortality of 0% was observed in the control tank at the end of the test period. This is within the range of mortality acceptable for validation of the test results.

The temperature of the control and test tanks was slightly above the recommended limit towards the end of the test, but as there was no pattern of increase in temperature, this was not thought to have adversely affected the data.