Reaction mass of 1,3-dichloropropene and 1,2-dichloropropane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to O.E.C.D. Test Guideline No. 202 with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(1E)-1,3-dichloroprop-1-ene; 1,2-dichloropropane

EC Number:

904-304-2

Molecular formula:

Multi-constituent: C3H4Cl2 and C3H6Cl2

IUPAC Name:

(1E)-1,3-dichloroprop-1-ene; 1,2-dichloropropane

Constituent 2

Reference substance name:

Crude 1,3-dichloropropene

IUPAC Name:

Crude 1,3-dichloropropene

Details on test material:

As per IUCLID5 Sections 1.1. - 1.4.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Details on test solutions:

A concentrated stock solution of the test substance was be prepared as appropriate in defined Elendt M4 dilution medium. This stock solution was be diluted to achieve the required nominal test substance concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia magna (Straus) strain held at Huntingdon Life Sciences was supplied by Sheffield University, having been originally obtained from the National Institute for Applied Chemical Research (IRCHA), France. Daphnia cultures were established with juvenile Daphnia taken from existing cultures after the first brood had been removed and discarded. Cultures are maintained in defined Elendt M4 dilution medium (between 500 and 1500 mL) in glass beakers with glass or perspex covers. The cultures were maintained in a temperature controlled laboratory at 20 +/- 2 degrees C. The day-length was controlled giving a photoperiod of 16 hours light, provided by overhead fluorescent tubes, and 8 hours dark. Daphnia cultures were fed daily with suspensions of the unicellular green alga

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

Hardness:

no data

Test temperature:

20 +/- 2 degrees C

pH:

No data

Dissolved oxygen:

> 3 mg/L

Salinity:

Freshwater

Nominal and measured concentrations:

Nominal test substance concentrations were: 0, 0.4.3, 0.94, 2.1, 4.55 and 10 mg/L

Details on test conditions:

The prepared test substance dilutions in defined medium were be placed into the test vessels. The test vessels were not aerated during the test. The vessels were of sufficient size to meet the minimum guideline criterion of one Daphnia per 2 mL of test substance dilution. Twenty Daphnia, less than 24 hours old, were used at each test substance concentration in the definitive test. Five Daphnia were placed in each vessel. The Daphnia were not fed during the exposure. Observations were made after 24 and 48 hours of exposure and the numbers of floating, mobile and immobile Daphnia were recorded. For the test to be valid, the number of floating and/or immobile Daphnia in the control group must not exceed 10% at the end of the test.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 5.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

immobilization

Remarks on result:

other: 4.3-6.1 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 2.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks:

immobilization

Remarks on result:

other: 2.35-3.34 mg/L

Details on results:

Significant complete immobilization (20/20) was observed at a nominal Crude 1,3-dichloropropene concentration of 10 mg/L at 24 hr of exposure. Complete immobilization of the Daphnids was observed at 4.55 and 10 mg/L following 48 hr of exposure. Immobilization was 1/20 at 2.1 mg/L and 6/20 at 4.55 mg/L at 24 hr. The immobilization was 1/20 at 0.94 and 2.1 mg/L after 48 hr of exposure.

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

Apprpriate statistical methods including 1-tailed Fisher's exact test were used to determine significance, estimate EC50 values and to calculate the 95% Confidence Intervals.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Crude 1,3-dichloropropene exposure induced an EC50 value of 2.8 mg/L (2.35-3.34 mg/L 95% Confidence Limits) in Daphnia following 48 hr of exposure based on immobilization. This finding suggests that Crude 1,3-dichloropropene is "toxic" to aquatic organisms.

Executive summary:

Crude 1,3-dichloropropene was assessed for acute toxicity to the water flea Daphnia magna in an O.E.C.D. Test Guideline No. 202 study with GLP compliance. Complete immobilization of the Daphnids was observed at 4.55 and 10 mg/L after 48 hr of exoposure. Crude 1,3-dichloropropene induced an EC50 value of 2.8 mg/L in Daphnia following 48 hr of exposure based on immobilization. This finding suggests that Crude 1,3-dichloropropene is "toxic" to aquatic organisms.