cis-hex-3-en-1-yl methyl carbonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25 June 2015 - 23 July 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Version / remarks:

July 17, 1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: "Testing Methods for New Chemical Substances" Japan (March 31, 2011, No. 0331-7; March 29, 2011, No.5; No. 110331009; April 2, 2012 partial revision, No. 0402-1; March 28, 2012, No.2; No. 120402001)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

ThOD = 60.6 mg O2 per 30 mg test substance added

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge: On-site sludge sampling was carried out at 10 locations in Japan (samples were from surface water and surface soil of rivers, lakes, and inland sea; rettun sludge from sewage plants)
- Storage length: sampling period: May, 2015, initiation date of use: June 19, 2015
- Preparation of inoculum for exposure: The activated sludge, which was cultivated for 19 hours after the synthetic sewage was added, was used. The synthetic sewage was prepared according to the following method; glucose, peptone, and potassitun dihydrogenphosphate were dissolved in purified water, and the pH of the solution was adjusted to 7.0 ± 1.0.
- Concentration of sludge: 3050 mg/L suspended solid in the activated sludge

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

BOD is measured

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD 301C
- Test temperature: 25 +/- 1 ºC
- pH: at start: 5.9-7.0, at the end: 6.2-7.3
- Aeration of dilution water: not indicated
- Suspended solids concentration: 30 mg/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus
- Number of culture flasks/concentration: 3 (sludge + test item)
- Method used to create aerobic conditions: stirring
- Measuring equipment: a closed system oxygen consumption measuring apparatus, which uses Soda lime No.1 for absorption of carbon dioxide

SAMPLING
- Sampling frequency: continuously
- Sampling method: BOD of the test solutions was measured continuously

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, one replicate
- Abiotic sterile control: water + test item: 1 replicate
- Reference control: sludge + aniline: 1 replicate
- toxicity control: no

Reference substance:

aniline

Remarks:

ThOD = 72.3 mg O2 per 30 mg substance added

Key result

Parameter:

% degradation (O2 consumption)

Value:

96 - 105

Sampling time:

28 d

Details on results:

average % biodegradation by BOD was 101
average % biodegradation by DOC was 98
average % decrease of test item (by GC) was 100

At day 7 and day 14, the % degradation of the test substance was already 73-78 and 89-95, resp.

Test solution (water +test item)

From the results of the quantitative analyses in the test solution after 28 days, the percentage residue of the test item was 10%, and the percentage production of cis-3-hexen-1-ol and methanol were 86% and 86%, respectively. It shows that the test item was hydrolyzed. 'The mass balances, which were calculated from these analytical results, the cis-3-hexen-1-ol part and methanol part were 96% and 96%, respectively. These results show that no other converted products except cis-3-hexen-1 -ol, methanol and carbon dioxide were produced. In addition, it was considered that residual amount of DOC derived from the test item methanol, and cis-3-hexen1-ol which were dissolved in the test solution.

Validity criteria fulfilled:

yes

Remarks:

see overall remarks

Interpretation of results:

readily biodegradable

Conclusions:

The substance showed 101% biodegradation in the modified MITI test (OECD 301C) and is considered readily biodegradable.

Executive summary:

The ready biodegradability of Liffarome was investigated in a study conducted in accordance with OECD 301C and GLP. The concentration tested was 100 mg/L test substance, with an activated sludge concentration of 30 mg/L. The test substance biodegraded for 76%, 93% and 101% after 7, 14 and 28 days, respectively, and is thus considered to be readily biodegradable. Cis-hexenol and methanol were detected as remaining metabolites.

Description of key information

The ready biodegradability of Liffarome was investigated in a study conducted in accordance with OECD 301C and GLP. The concentration tested was 100 mg/L test substance, with an activated sludge concentration of 30 mg/L. The test substance biodegraded for 76%, 93% and 101% after 7, 14 and 28 days, respectively, and is thus considered to be readily biodegradable. Cis-hexenol and methanol were detected as remaining metabolites.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable