Iron oxide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Guideline study with acceptable restrictions. Iron(II)Oxide belongs to the iron oxides family and presents comparable physico chemical properties, leading to similar behaviour for the all members of the iron oxide substances. In particular, based on affinity of the chemical structure, Iron Oxide (Fe3O4) and Iron(III)Oxide (Fe2O3) are selected to support read across for the Iron(II)Oxide dossier. Full justification paper is provided in the current Summary Section.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Proposed procedure by the Federal Environmental Agency (Umweltbundesamt) for lethal effect on Zebrabaerbling, (May 1984), LC0, LC50, LC100, 48-96h

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diiron trioxide

EC Number:

215-168-2

EC Name:

Diiron trioxide

Cas Number:

1309-37-1

Molecular formula:

Fe2O3

IUPAC Name:

diiron trioxide

Constituent 2

Reference substance name:

215-186-2

IUPAC Name:

215-186-2

Constituent 3

Reference substance name:

Iron(III)oxide

IUPAC Name:

Iron(III)oxide

Details on test material:

1 kg of Bayferrox 130 red was mixed with 10 l of water, shaken for 24 h and filtered through a paper filter. The eluate was used in dulutions 1:10 (10 g/L), 1:5 (20 g/L), 1:2 (50 g/L) and undiluted (100 g/L).

Test solutions

Details on test solutions:

- 4 concentrations tested: 100000, 50000, 20000, and 10000 mg/l. The test concentrations were achieved by dilution

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Dilution water: synthetic freshwater according to IDSO
Water hardness: 15°dH

Test temperature:

23 +- 2 °C

pH:

6.2 - 7.1 (6.3 - 6.8 in dilution water)

Dissolved oxygen:

5.0 . 8.3 mg/L (Oxygen >= 90% saturation)

Salinity:

- Ca:Mg ratio: 4:1

Details on test conditions:

- Application of test substance: stock solution stirred for 24 h and then filtered through a paper filter
- Aeration: gentle aeration via narrow glass tubes
- Loading rate: 10 animals/vessel

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 50 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

other: LC90

Effect conc.:

ca. 100 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

Within 96 hours no effect (LC0) on Brachydanio rerio was observed up to a concentration of >= 50000 mg/l, and a 96-hour LC90 of ca. 100000 mg/l was obtained. The method used was a proposed procedure for lethal effect on Zebrabaerbling. (Federal Environmental Agency Germany May 1984), (Bayer AG 1989).