Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 231-445-0 | CAS number: 7554-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: oral
Administrative data
- Endpoint:
- short-term repeated dose toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Animal experimental study, published in peer reviewed literature, restrictions in design and/or reporting but otherwise adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Long term toxicity study with alcohol and 4-methylpyrazole in rat.
- Author:
- Kager L.
- Year:
- 1 974
- Bibliographic source:
- Acta Pathol Microbiol Scand A. 1974 Jul;82(4):534-8.
Materials and methods
- Principles of method if other than guideline:
- Twelve male Sprague-Dawley rats were exposed to the test substance continuously via drinking water for 12 weeks. During the exposure period clinical signs and fluid consumption were recorded. Animals were weighed once a week. After the exposure period blood was taken for haematology and clinical chemistry, and animals were necropsied.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- 4-methylpyrazole
- EC Number:
- 231-445-0
- EC Name:
- 4-methylpyrazole
- Cas Number:
- 7554-65-6
- Molecular formula:
- C4H6N2
- IUPAC Name:
- 4-methyl-1H-pyrazole
- Details on test material:
- Name of test material (as cited in study article): 4-methylpyrazole
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: AB Anticimex (Sollentuna, Sweden)
- Age at study initiation: 5-6 weeks
- Weight at study initiation: mean 130 g
- Housing: individually
- Diet: ad libitum, Anticimex Mouse and Rat Food 201 (3.7% fat, 37.2% protein)
- Water: ad libitum
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- water
- Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- 12 weeks
- Frequency of treatment:
- continuously
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0.13 ± 0.02 mmol/kg bw
Basis:
actual ingested
- Remarks:
- Doses / Concentrations:
14.8 ± 2.4 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 12 male animals
- Control animals:
- yes, concurrent vehicle
Examinations
- Observations and examinations performed and frequency:
- -CAGE SIDE OBSERVATIONS: were performed, frequency unknown
-BODY WEIGHT: once a week rats were weighed.
-WATER CONSUMPTION AND COMPOUND INTAKE: Amounts of fluid consumed were registered once a week.
-HAEMATOLOGY: At termination of the experiments, after 12 weeks, the aorta was catheterized under ether anaesthesia. Determinations of hemoglobin, leucocytes, differential white cell count.
-CLINICAL CHEMISTRY: At termination of the experiments, after 12 weeks, the aorta was catheterized under ether anaesthesia. Determination of serum creatinin, GPT, GOT and serum protein. - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes; liver, kidneys, heart and spleen were weighed.
HISTOPATHOLOGY: Liver, pancreas, kidney, and heart were taken for microscopic examination.
Results and discussion
Results of examinations
- Details on results:
- In all animals treated with 4-methylpyrazole no clinical symptoms and no signs of toxicity occurred. Body weight gain and organ weights were comparable to the concurrent control. There were no changes in liver function and the results of the haematological investigations revealed no signs of damage to the bone marrow. There were no changes of gross pathology and histology.
Effect levels
- Dose descriptor:
- NOEL
- Effect level:
- 14.8 mg/kg bw/day (actual dose received)
- Based on:
- test mat.
- Sex:
- male
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.