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EC number: 231-445-0 | CAS number: 7554-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant guideline study, available as unpublished report, no restrictions, fully adequate for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 4-methylpyrazole
- EC Number:
- 231-445-0
- EC Name:
- 4-methylpyrazole
- Cas Number:
- 7554-65-6
- Molecular formula:
- C4H6N2
- IUPAC Name:
- 4-methyl-1H-pyrazole
- Details on test material:
- - Name of test material (as cited in study report): 4-Methylpyrazol
- Description: pale yellow liquid
- Storage conditions: room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Iffa Crédo, 69210 L'Arbresle, France
- Age at study initiation: aprroximately 8 weeks
- Weight at study initiation: mean 259 ± 7 g for males, and 227 ± 8 g for females
- Housing: during the acclimatization period animals were housed 1-7 animals per cage, in polycarbonate cages (48 cm x 27 cm x 20 cm); during the treatment animals were housed individually, in polycarbonate cages (35.5 cm x 23.5 cm x 19.3 cm); each cage contained dust-free sawdust (SICSA, 94142 Alfortville, France)
- Diet: ad libitum, A04C pelleted diet (UAR, 91360 Villemoisson-sur-Orge, France)
- Water: ad libitum, filtered water by a FG Millipore membrane (0.22 micron)
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 ± 2
- Humidity (%): 30 - 70
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure and preparation: on the day before treatment, the dorsal area (6 cm x 8 cm) of each animals was clipped.
- % coverage: it was placed on an area representing 10% (5 cm x 6 cm for the females, and 5 cm x 7 cm for the males) of the body surface of the animals
- Type of wrap if used: a hydrophylic gauze pad, which was held in contact with the skin by means of an adhesive hypoallergenic aerated semi-occlusive dressing and restraining bandage.
REMOVAL OF TEST SUBSTANCE
- No residual test substance was observed at removal of the dressing.
- Time after start of exposure: 24 hours
TEST MATERIAL
- Amount(s) applied: 0.41, 1, 2.02 mL/kg (based on a specific gravity of 0.993 g/mL) - Duration of exposure:
- 24 hours
- Doses:
- 400, 1000, 2000 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- other: historical control
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed frequently during the hours following administration. Thereafter, observation of the animals was made at least once a day until day 15. From day 2, only local cutaneous reaction was recorded. Animals were weighed individually just before administration on day 1, and on days 8 and 15.
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 1 000 - < 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- - No mortality was observed in animals exposed to 400 and 1000 mg/kg.
- All animals exposed to 2000 mg/kg died between day 2 and day 13 following treatment. - Clinical signs:
- other: - 400 mg/kg dose group: Sedation was recorded in all animals on days 1 and 2. Ocular secretion was noted in 2 out of 5 females on day 2. No clinical signs persited on day 3. - 1000 mg/kg dose group: Sedation was observed in all animals on days 1 and 2, an
- Gross pathology:
- Macroscopic examination of the main organs revealed no apparent abnormalities.
- Other findings:
- No cutaneous reactions were recorded in all exposed animals.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information
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