2H-Azepin-2-one, hexahydro-, polymer with 1,6-diisocyanatohexane

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002-07-18 to 2002-09-13

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Directive 92/69/EEC

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Analysis at beginning and end of test (0 and 48 h)

Vehicle:

no

Details on test solutions:

- Concentration: 1 g/l stirred in deionized water for 18 hours and filtered, no vehicle or solvent; analytical concentration 0.37 mg/l

Test organisms (species):

Daphnia magna

Details on test organisms:

- Strain: Daphnia magna Straus, clone 5
- Source/supplier: Infracor GmbH (inhouse)
- Breeding method: In 1 l beakers with M4 medium (Elendt,
1990), water renewal each 2-3 days, isolation of offspring
for further breeding each ca. 4 weeks
- Age: < 24 hours
- Feeding: Desmodesmus subspicatus, daily as much as
consumed
- Pretreatment: Separation from adults 24 h prior to testing
- Feeding during test: none
- Control group: 2 reference substance controls (1.0 and 2.0
mg/l), one blank

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 d

Test temperature:

20.16 - 21.54 (mean 20.41) degree C

pH:

7.7 - 7.8

Dissolved oxygen:

7.6 - 8.0 mg/l

Nominal and measured concentrations:

- Concentrations: (analytical) 0.33 mg/l (0 hours) / 0.36 mg/l (48 hours)

Details on test conditions:

TEST SYSTEM
- Exposure vessel type: round-bottom test tubes with 10 ml
- Number of replicates, individuals per replicate:
4 replicates with 5 individuals each

DILUTION WATER
- Source: Synthetic:
CaCl2 x 2 H2O: 294 mg/l
MgSO4 x 6 H2O: 114 mg/l
NaHCO3: 65 mg/l
KCl: 6 mg/l
- Ca/Mg ratio: 4:1
- Na/K ratio: 10:1
- Hardness: 14 degree dH = 250 mg CaCO3/l
- Aeration: none

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.33 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

At the highest concentration which could be achieved under the conditions (water solubiilty limit) of the test no negative effect could be observed.

- Effect data (Immobilisation): No. of immobile daphnia
Control: 0 (24 h) / 0 (48 h)
Exposed: 0 (24 h) / 0 (48 h)

Results with reference substance (positive control):

- Concentrations: 1.0 mg/l / 2.0 mg/l
- Results: 35 % immobile / 100 % immobile (24 hours)

Validity criteria fulfilled:

yes

Conclusions:

No effects were observed up to the water solubility limit (0.33 mg/L) indicating that 2H-Azepin-2-one, hexahydro-, polymer with 1,6-diisocyanatohexane does not pose a significant hazard to aquatic invertebrates.

Executive summary:

The acute toxicity of 2H-Azepin-2-one, hexahydro-, polymer with 1,6-diisocyanatohexane to Daphnia magna was studied under static conditions according to OECD TGD 202 and EU method C2. Daphnids were exposed to the test substance at a saturated stock solution for 48 h. No effects were observed up to the water solubility limit (0.33 mg/L) and thus the EC50 was determined to be >0.33 mg/L. This indicates that the test substance does not pose a significant hazard to aquatic invertebrates. The study was assessed as "reliable without restrictions".

Description of key information

The acute toxicity of the test substance to Daphnia magna was studied under static conditions according to OECD TGD 202 and EU method C2. Daphnids were exposed to the test substance at a saturated stock solution for 48 h. No effects were observed up to the water solubility limit (0.33 mg/L) and thus the EC50 was determined to be >0.33 mg/L. This indicates that the test substance does not pose a significant hazard to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Remarks:

limit of water solubility

Effect concentration:

> 0.33 mg/L