2-tert-butylhydroquinone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

Toxicity of test chemical on the microorganisms were studied by using Agar-plate test.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Solvent used: water or acetone

Test organisms (species):

other: Gloeophyllum trabeum ATCC 11539

Details on inoculum:

Common name: Brown-rot fungus

Test type:

static

Water media type:

not specified

Remarks on exposure duration:

Exposure duration not reported

Nominal and measured concentrations:

25 - 500 ppm

Details on test conditions:

five replicates were used; radial fungal growth normalized to fungal growth on control plates was observed; IC50 value was calculated by regression of relative growth versus log of concentration

Reference substance (positive control):

not specified

Key result

Dose descriptor:

IC50

Effect conc.:

< 200 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Exposure duration not reported; Reported value: <200ppm

Validity criteria fulfilled:

no

Conclusions:

The IC50 value of test chemical for Gloeophyllum trabeum ATCC 11539 is observed to be < 200 mg/L.

Executive summary:

Toxicity of test chemical on the microorganisms were studied by using Agar-plate test. Test conducted under the static system. Five replicates were used; radial fungal growth normalized to fungal growth on control plates was observed; IC50 value was calculated by regression of relative growth versus log of concentration. The IC50 value of test chemical for Gloeophyllum trabeum ATCC 11539 is observed to be < 200 mg/L.

Description of key information

Toxicity of test chemical on the microorganisms were studied by using Agar-plate test. Test conducted under the static system. Five replicates were used; radial fungal growth normalized to fungal growth on control plates was observed; IC50 value was calculated by regression of relative growth versus log of concentration. The IC50 value of test chemical for Gloeophyllum trabeum ATCC 11539 is observed to be < 200 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

200 mg/L

Additional information

Summarized result from various sources and peer reviewed journal were studied and mention as below:

In the first study toxicity of test chemical on the microorganisms were studied by using Agar-plate test. Test conducted under the static system. Five replicates were used; radial fungal growth normalized to fungal growth on control plates was observed; IC50 value was calculated by regression of relative growth versus log of concentration. The IC50 value of test chemical for Gloeophyllum trabeum ATCC 11539 is observed to be < 200 mg/L.

 

Toxicity study under the static system was performed on White-rot fungus. Test solution prepared by using acetone as a vehicle. The IC50 value of test chemical for Trametes versicolor ATCC 12679 is < 200 mg/L.

 

Above study was supported by the third study from peer reviewed journal. Static test for 5 weeks were performed on Gloeophyllum trabeum ATCC 11539. Laboratory decay test; soil-block test; SYP sapwood wafers treated with test chemical and exposed to the brown-rot fungus for 5 weeks. Extent of degradation measured by compression strength loss in radial direction. Treatment with concentration of 5 % were used. In Gloeophyllum trabeum ATCC 11539 (brown-rot fungus), treatment with test chemical for 35 days, results in average percent strength loss of 9.2% as compared to 83.0-99.1% in control at 5% concentration of test chemical.