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EC number: 265-019-0 | CAS number: 64690-19-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental start date 13 March 2018, Experimental completion date 25 April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- yes
- Remarks:
- On Day 14 of the study the flow rate for Inoculum Control Replicate 1 was measured to be 20 mL/minute.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-octylpyridin-4-amine
- EC Number:
- 265-019-0
- EC Name:
- N-octylpyridin-4-amine
- Cas Number:
- 64690-19-3
- Molecular formula:
- C13H22N2
- IUPAC Name:
- N-octylpyridin-4-amine
Constituent 1
- Specific details on test material used for the study:
- Test Item and Supporting Information
Information as provided by the Sponsor.
Identification: N-Octyl-4-pyridinamine
Batch: 80030735B
Purity: not supplied
Physical state/Appearance: white solid
Expiry Date: 28 November 2018
Storage Conditions: room temperature in the dark
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic (adaptation not specified)
- Details on inoculum:
- A mixed population of activated sewage sludge micro organisms was obtained on 12 March 2018 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.
- Duration of test (contact time):
- 29 d
Initial test substance concentration
- Initial conc.:
- 13.2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 13
- Sampling time:
- 28 d
Any other information on results incl. tables
Validation Criteriaand Biodegradation
The total CO2evolution in the inoculum control vessels on Day 28 was 33.10 mg/L and therefore satisfied the validation criterion given in the OECD Test Guidelines.
The IC content of the test item suspension in the mineral medium at the start of the test was below 5% of the TC content and hence satisfied the validation criterion given in the OECD Test Guidelines.
The difference between the values for CO2production at the end of the test for the replicate vessels was <20% and hence satisfied the validation criterion given in the OECD Test Guidelines.
Acidification of the test vessels on Day 28 followed by the final analyses on Day 29 was conducted according to the methods specified in the Test Guidelines. This acidification effectively kills the micro‑organisms present and drives off any dissolved CO2present in the test vessels. Therefore any additional CO2detected in the Day 29 samples originated from dissolved CO2that was present in the test vessels on Day 28 and hence the biodegradation value calculated from the Day 29 analyses is taken as being the final biodegradation value for the test item.
The results of the inorganic carbon analysis of samples from the first absorber vessels on Day 29 showed an increase in all replicate vessels with the exception of inoculum control Replicate 1 and procedure control Replicate 2.
Inorganic carbon analysis of the samples from the second absorber vessels on Day 29 confirmed that no significant carry-over of CO2into the second absorber vessels occurred.
The test item attained 13% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
Sodium benzoate attained 64% biodegradation after 14 days with greater than 60% degradation being attained in a 10-Day window. After 28 days 84% biodegradation was attained. These results confirmed the suitability of the inoculum and test conditions and satisfied the validation criterion given in the OECD Test Guidelines.
The toxicity control only attained 22% biodegradation after 14 days and 38% biodegradation after 28 days thereby confirming that the test item exhibited inhibitory effect on the sewage treatment micro‑organisms used in the test.
Therefore, care should be taken in the interpretation of these results as the toxicity control vessel did not pass the validation criteria whereby it should attain equal to or greater than 25% biodegradation by Day 14 of the study for the test item to be considered non-inhibitory.
Day |
Biodegradation |
||
Procedure Control |
Test Item |
Toxicity Control |
|
0 |
0 |
0 |
0 |
2 |
20 |
0 |
19 |
6 |
23 |
0 |
17 |
8 |
28 |
0 |
19 |
10 |
55 |
0 |
19 |
14 |
64 |
0 |
22 |
21 |
78 |
1 |
36 |
28 |
82 |
8 |
35 |
29* |
84 |
13 |
38 |
*Day 29 values corrected to include any carry-over of CO2detected in Absorber 2
Conclusion
The test item attained 13% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
The reference item, Sodium benzoate attained 64% biodegradation after 14 days with greater than 60% degradation being attained in a 10-Day window. After 28 days 84% biodegradation was attained. These results confirmed the suitability of the inoculum and test conditions and satisfied the validation criterion given in the OECD Test Guidelines.
The toxicity control only attained 22% biodegradation after 14 days and 38% biodegradation after 28 days thereby confirming that the test item exhibited inhibitory effect on the sewage treatment micro‑organisms used in the test.
Therefore, care should be taken in the interpretation of these results as the toxicity control vessel did not pass the validation criteria whereby it should attain equal to or greater than 25% biodegradation by Day 14 of the study for the test item to be considered non-inhibitory.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- no
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item attained 13% biodegradation after 28 days and therefore cannot be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No. 301B.
The reference item, Sodium benzoate attained 64% biodegradation after 14 days with greater than 60% degradation being attained in a 10-Day window. After 28 days 84% biodegradation was attained. These results confirmed the suitability of the inoculum and test conditions and satisfied the validation criterion given in the OECD Test Guidelines.
The toxicity control only attained 22% biodegradation after 14 days and 38% biodegradation after 28 days thereby confirming that the test item exhibited inhibitory effect on the sewage treatment micro-organisms used in the test.
Therefore, care should be taken in the interpretation of these results as the toxicity control vessel did not pass the validation criteria whereby it should attain equal to or greater than 25% biodegradation by Day 14 of the study for the test item to be considered non-inhibitory.
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