Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-532-9 | CAS number: 142-29-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in Section 13.
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Reference
- Endpoint:
- skin irritation: in vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Principles of method if other than guideline:
- BASF test:
ca. 1 ml of the undiluted test substance was applied to a 2.5 x 2.5 cm application site of two white Vienna rabbits for 20 h under occlusive conditions. The skin was shaved but intact. The two animals were observed for 8 days and skin changes were observed on working days. Findings were recorded after 24, 48 hours as well as on day 5, 7 and at the end of the observation period on day 8. Findings were graded as described in OECD test guideline 404. - GLP compliance:
- no
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: 2.99 and 2.54 kg
- Diet: ad libitum
- Water: ad libitum - Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): ca. 1 ml - Duration of treatment / exposure:
- 20 hours
- Observation period:
- 8 days
- Number of animals:
- 2
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 X 2.5 cm, shaved but intact skin
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing done
SCORING SYSTEM:
BASF scheme
Erythema:
(+) corresponds to Draize score 1
R+ corresponds to Draize score 2
R++ corresponds to Draize score 3
R+++ corresponds to Draize score 4
Edema
Ö(+) corresponds to Draize score 1
Ö+ vorresponds to Draize score 2
Ö++ corresponds to Draize score 3
Sack Ö+++ corresponds to Draize score 4
Sch+ slight scales
Sch++ rhagade-like scales
Readings were performed 24 hours, 48 hours 5, 7 and 8 days after the application of the test substance. - Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.75
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days (only animal 1)
- Remarks on result:
- other: 72 hr time endpoint not measured
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days
- Remarks on result:
- other: for animal 1 the 5 day score was considered as edema was not graded after 48 hours; slight scaling was observed after 7 and 8 days
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- The test material was applied for 20 hours under occlusive conditions. The result thus represents a worst case. As irritation was still observed at the end of the observation period in 50 % of the animals and slight scaling was observed in both animals, the substance is regared as skin irritant Cat. 2 as a worst case assumption although the 24/48 hour values alone would not warrant classification according to CLP.
- Executive summary:
In this study, 1 mL of a test material, cyclohexene was applied to skin of two rabbits for 20 hours under occlusive conditions.
The mean erythema score obtained was 1.75 and the mean oedema score was 2, which were not reversible within the 8 -day observation period.
As irritation was still observed at the end of the observation period in 50 % of the animals and slight scaling was observed in both animals, the substance is regared as skin irritant Cat. 2 as a worst case assumption although the 24/48 hour values alone would not warrant classification according to CLP.
|
Animal 1 |
Animal 2 |
Mean |
|||
|
Erythema |
Ed. |
Erythema |
Edema |
Ery. |
Ed. |
24 hours |
1 |
2 |
2 |
2 |
1.5 |
2 |
48 hours |
2 |
unknown |
2 |
2 |
2 |
2 |
5 days |
2 |
2 |
2 |
2 |
2 |
2 |
7 days |
2 |
slight scaling |
0 |
slight scaling |
1 |
- |
8 days |
1
|
slight scaling |
0 |
slight scaling |
0.5 |
- |
Mean 24 and 48 hours |
1.5 |
- |
2 |
2 |
|
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 973
- Report date:
- 1973
Materials and methods
- Principles of method if other than guideline:
- BASF test:
ca. 1 ml of the undiluted test substance was applied to a 2.5 x 2.5 cm application site of two white Vienna rabbits for 20 h under occlusive conditions. The skin was shaved but intact. The two animals were observed for 8 days and skin changes were observed on working days. Findings were recorded after 24, 48 hours as well as on day 5, 7 and at the end of the observation period on day 8. Findings were graded as described in OECD test guideline 404. - GLP compliance:
- no
Test material
- Reference substance name:
- Cyclohexene
- EC Number:
- 203-807-8
- EC Name:
- Cyclohexene
- Cas Number:
- 110-83-8
- Molecular formula:
- C6H10
- IUPAC Name:
- cyclohexene
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Gaukler
- Weight at study initiation: 2.99 and 2.54 kg
- Diet: ad libitum
- Water: ad libitum
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): ca. 1 ml - Duration of treatment / exposure:
- 20 hours
- Observation period:
- 8 days
- Number of animals:
- 2
- Details on study design:
- TEST SITE
- Area of exposure: 2.5 X 2.5 cm, shaved but intact skin
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing done
SCORING SYSTEM:
BASF scheme
Erythema:
(+) corresponds to Draize score 1
R+ corresponds to Draize score 2
R++ corresponds to Draize score 3
R+++ corresponds to Draize score 4
Edema
Ö(+) corresponds to Draize score 1
Ö+ vorresponds to Draize score 2
Ö++ corresponds to Draize score 3
Sack Ö+++ corresponds to Draize score 4
Sch+ slight scales
Sch++ rhagade-like scales
Readings were performed 24 hours, 48 hours 5, 7 and 8 days after the application of the test substance.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.75
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days (only animal 1)
- Remarks on result:
- other: 72 hr time endpoint not measured
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 4
- Reversibility:
- not fully reversible within: 8 days
- Remarks on result:
- other: for animal 1 the 5 day score was considered as edema was not graded after 48 hours; slight scaling was observed after 7 and 8 days
- Remarks:
- 72 hr time endpoint not measured
Any other information on results incl. tables
|
Animal 1 |
Animal 2 |
Mean |
|||
|
Erythema |
Ed. |
Erythema |
Edema |
Ery. |
Ed. |
24 hours |
1 |
2 |
2 |
2 |
1.5 |
2 |
48 hours |
2 |
unknown |
2 |
2 |
2 |
2 |
5 days |
2 |
2 |
2 |
2 |
2 |
2 |
7 days |
2 |
slight scaling |
0 |
slight scaling |
1 |
- |
8 days |
1
|
slight scaling |
0 |
slight scaling |
0.5 |
- |
Mean 24 and 48 hours |
1.5 |
- |
2 |
2 |
|
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritant) based on GHS criteria
- Conclusions:
- The test material was applied for 20 hours under occlusive conditions. The result thus represents a worst case. As irritation was still observed at the end of the observation period in 50 % of the animals and slight scaling was observed in both animals, the substance is regared as skin irritant Cat. 2 as a worst case assumption although the 24/48 hour values alone would not warrant classification according to CLP.
- Executive summary:
In this study, 1 mL of a test material, cyclohexene was applied to skin of two rabbits for 20 hours under occlusive conditions.
The mean erythema score obtained was 1.75 and the mean oedema score was 2, which were not reversible within the 8 -day observation period.
As irritation was still observed at the end of the observation period in 50 % of the animals and slight scaling was observed in both animals, the substance is regared as skin irritant Cat. 2 as a worst case assumption although the 24/48 hour values alone would not warrant classification according to CLP.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.