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EC number: 204-854-7 | CAS number: 127-65-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP test according to protocol. No analyses were performed, but from a stability test it is knwn that the test substance is stable in algae medium.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The NaHCO3 concentration was increased from 50 to 150 mg/L. The temperature was out of range for a few hours at the beginning of the test. The ammoniumchloride was replaced by sodiumnitrate as sole source of nitrogen to increase the stability of the test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- - Source/supplier: CCAP, Cumbria, UK
- Laboratory culture: yes
- Method of cultivation: batch cultures
- Pretreatment: no
- Controls: yes
- Initial cell concentration: 1 x 10^4 cells/ml - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21.1 - 24.0 °C
- pH:
- 7.8 - 9.3
- Nominal and measured concentrations:
- - Nominal concentrations: 0.2, 0.6, 1.8, 5.4, 16.1 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 ml Erlenmeyer flasks
- Aeration: no
- Initial cells density: 1x10E4
- Control end cells density: 3.2x10E6
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes, OECD medium, with 150 mg/l NaHCO3 instead of 50 mg/l and the ammoniumchloride was replaced by sodiumnitrate as sole source of nitrogen to increase the stability of the test compound during the test periode.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: see growth medium
- Culture medium different from test medium: No
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: continuous light
- Light intensity and quality: spectral range of 400 to 700 nm by using 30 W fluorescent lamps of the type ‘universal white’, 103.81 and
104.70 µmol/m²/s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer - Reference substance (positive control):
- yes
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: both biomass and growth rate
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: both biomass and growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- - Substance: potassium chromate
- Concentrations: 0.1-0.25-0.63-1.56-3.91 mg/l
- Results: EbC50 = 0.75 and ErC50 is 2.0 mg/l - Reported statistics and error estimates:
- NOEC and LOEC were determined using William's test (Williams, D.A., Biometrics 28, pp. 519-531 (1972)) ). Confidence limits were based on Fieller's theorem (Zerbe, G.O., Statistician, Vol 32 (3).pp. 103-105)
- Validity criteria fulfilled:
- yes
- Conclusions:
- Validity criteria were fulfilled.
The EbC50 and ErC50 (0-96 h) of Tosylchloramide sodium are 4.5 (95% CL 3.9-5.2) and 13 (0 95% CL 11.0-16.), respectively. The LOEC determined from the results for Tosylchloramide sodium is 0.6 mg/L. The NOEC derived from the results for Chloramine-T is 0.2 mg/L. - Executive summary:
In order to predict effects of chemicals in an aquatic environment, the toxicity to algae was assessed. The algal toxicity was determined in the Algal Growth Inhibition test in accordance with slightly modified OECD Test Guidelines (which is basically identical to the EEC protocol and IS0 guidelines), and in compliance with the OECD Principles of Good Laboratory Practice.
The toxicity of Tosylchloramide sodium to exponentially growing Selenastrurn capricornutum was determined over an exposure period of 96 hours. The test was conducted in a mineral salts medium at temperatures ranging from 21.1 to 24.0°C in an illuminated orbital incubator. The pH in the test media varied from 7.8 to 9.3.
The definitive test was carried out at nominal concentrations of 0.2, 0.6, 5.4 and 16.1 mg/l Chloramine-T. The EbC50 and ErC50 (0-96 h) of Tosylchloramide sodium are 4.5 mg/l (3.9 -5.2 95% confidence limits) and 13 mg/l (11.0-16.0 95% confidence limits), respectively. The LOEC determined from the results for Tosylchloramide sodium is 0.6 mg/l. The NOEC derived from the results for Tosylchloramide sodium is 0.2 mg/l.
The definitive test is valid as shown by the EbC50 and ErC50 values of the reference compound potassium dichromate (0.75 and 2.0 mgll, respectively), the increase of the extinction of the control over 72 h by a factor of 74 and by a maximum deviation of the pH of 1.5 unit.
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1990
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- No data on the test substance other than name. No details on measurement only EC50s are presented in the article. Due to lack of data a dose-response relationship cannot be determined. No dose-effect data given. Stability of the test substance in the test medium is not provided. Test substance is not monitored during the study. High intial cell number. For meeting the quality criteria only the pH of the controls was measured and not the pH of the test vessels. Authors conclude that the criteria was met because the pH at the beginning of the test was 8.1 and after 48 h it was 9.6. No data for test vessels.
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 38 412, Part 9 (draft standard)
- Deviations:
- yes
- Remarks:
- slight modification
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.2 mg/L
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.58 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.31 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- biomass
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The ErC50 is 0.58 mg/L
The NOEC (based on growth rate) is 0.2 mg/L
Referenceopen allclose all
The cell concentration in control cultures increased at least by a factor of 16 within 3 days that validity criterion has been fulfilled. The concentration of the test substance was not determined therefore it cannot be assessed if the test substance concentration remains within 80% of the nominal concentration. A dose response relationship is not shown due to insufficient reporting of data. Only the ErC50 and EbC50 are given of 0.58 and 0.31 mg/L, respectively.
Description of key information
In order to predict effects of chemicals in an aquatic environment, the toxicity to algae was assessed. The algal toxicity was determined in the Algal Growth Inhibition test in accordance with slightly modified OECD Test Guidelines (which is basically identical to the EEC protocol and IS0 guidelines), and in compliance with the OECD Principles of Good Laboratory Practice. The toxicity of Tosylchloramide sodium to exponentially growing Selenastrurn capricornutum was determined over an exposure period of 96 hours. The test was conducted in a mineral salts medium at temperatures ranging from 21.1 to 24.0°C in an illuminated orbital incubator. The pH in the test media varied from 7.8 to 9.3. The definitive test was carried out at nominal concentrations of 0.2, 0.6, 5.4 and 16.1 mg/l Tosylchloramide sodium. The EbC50 and ErC50 (0-96 h) of Tosylchloramide sodium are 4.5 mg/l (3.9 -5.2 95% confidence limits) and 13 mg/l (11.0-16.0 95% confidence limits), respectively. The LOEC determined from the results for Tosylchloramide sodium is 0.6 mg/l. The NOEC derived from the results for Tosylchloramide sodium is 0.2 mg/l. The definitive test is valid as shown by the EbC50 and ErC50 values of the reference compound potassium dichromate (0.75 and 2.0 mg/l, respectively), the increase of the extinction of the control over 72 h by a factor of 74 and by a maximum deviation of the pH of 1.5 unit.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 13 mg/L
- EC10 or NOEC for freshwater algae:
- 3 mg/L
Additional information
The key value is derived from Kroon (1997) based on the reliability, duration and quality of the study. Although not calculated, it can be derived from the study (fig 4) that the ErC10 value could be between 3 - 4 mg/L (worst case 3 mg/L).
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