Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 230-939-3 | CAS number: 7378-99-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 - 29 Nov 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test item name: AD 1 Batch number. 4500083770EAS Test item code: M-00000740Appearance / colour: liquid / clearChemical name: N,N-dimethyloctylaminePurity (nominal): > 95 % w/w CAS number: 7378-99-6Molecular weight: 157.3 g/molRelative density (nominal): 0.765 g/cm³ Signal word(s): dangerIssue date of certificate: 21 Jan 2015Expiry date: 23 Sep 2016 Storage conditions: ambient ( ≤ +30 °C), dark, dry
- Analytical monitoring:
- yes
- Details on sampling:
- The analytical verification of test item concentrations in daphnid test medium was done by analysing the content of AD 1 in the samples during the test. Samples from control and all test item concentrations were analysed at test start t = 0 h from fresh test solutions and after 48 hours from aged test solutions.
- Vehicle:
- no
- Details on test solutions:
- The necessary amount of the test item for preparing the stock solution was weighed on weighing scoops and transferred to a volumetric flask. Test medium (see 4.3) was added up to the bench mark and the solution was homogenised by shaking. The solution was clear and an oily film was observed on the inner glass surfaces – this may have accounted for the lower actual (measured) concentrations. However, as a clear dose response was observed, study validity was not impacted. The lower test solutions were prepared by dilution of the stock solution. Defined volumes of the prepared solution were transferred to each test vessels. The test solution volume was 50 mL per test vessel.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- As test organism, Daphnia magna STRAUS, Clone V, was used. The animals are continuously bred in the laboratory and were originally purchased in a healthy condition from the Federal Environmental Agency in Berlin/Germany.Daphnia magna was bred as single culture (one daphnid per 100 mL) in Elendt M4 medium. The pH-value of the aerated water was within a range of 6.0 – 9.0. The dissolved oxygen was above 60 % saturation and the total hardness 140 - 250 mg/L (as CaCO3), corresponding to 7.8 - 14°dH. The animals were fed with single cell green algae (Desmodesmus subspicatus, formerly Scenedesmus subspicatus) at least three times a week.The daphnids were reared at a temperature of 20 2 °C in a climatic chamber with 16 h of illumination and 8 h of darkness. The medium was changed three times per week. A pipette was used to separate the young daphnids from the adults.Freshly hatched daphnids less than 24 hours old were used for the test.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 13 dH (232 mg/L CaCO3)
- Test temperature:
- 20.2 - 20.6 °C
- pH:
- 7.56 - 9.47
- Dissolved oxygen:
- ≥ 7.6
- Nominal and measured concentrations:
- see table below
- Details on test conditions:
- Test procedure:staticDuration:48 hoursTemperature:20.2 – 20.6 °COxygen concentration:7.6 – 8.9 mg/LpH-value:7.56 – 9.47Exposure to light:16 hours photoperiod / 8 hours darkness dailyFeeding:noneTest vessels:four 100 mL glass beakers per concentration, each filled with 50 mL; one additional replicate for physico-chemical parameters without test organismsLoading:10 mL of test solution for each animal Aeration:noneNumber of animals: 20 per concentration in 4 replicates of 5
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 22.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.26 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14.7 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 7.51 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.26 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.28 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Details on results:
- After 24h of exposure no immobilisation was observed in the control and up to and including the nominal concentration of 7.51 mg/L. 35 % immobilisation was observed at 16.5 mg/L and 75 % immobilisation was observed at 36.4 mg/L while mobile specimen showed a decrease in movement speed. At the highest test item concentration of 80.0 mg/L all daphnids were found immobile.After 48h of exposure no immobilisation was observed in the control and at a nominal concentration of 3.42 mg/L. At 7.51 mg/L no immobilisation higher than the allowed control immobilisation was observed. At 16.5 mg/L 55 % of the daphnids were found immobile. At 36.4 mg/L and the highest test item concentration of 80.0 mg/L all daphnids were found immobile.
- Results with reference substance (positive control):
- The results indicate an EC50 (24 h) of the reference item potassium dichromate between 1.0 and 2.0 mg/L in the reference test. Since the results fall within the historical data generated with the reference item at the testing facility and the range recommended by the guideline (OECD 202), the daphnids were suitable for the determination of the toxicological effects of the test item.
- Validity criteria fulfilled:
- yes
- Conclusions:
- According to the results of the test, the EC50 (48 h) for immobilisation was determined to be 14.5 mg/L (nominal) and 9.28 mg/L (actual).The corresponding NOEC (48 h) was determined to be 7.51 mg/L (nominal) and 5.26 mg/L (actual).
- Executive summary:
The initial measured concentration of AD 1 was between 55 and
88 % of nominal, the measured concentration in the aged solution was between 54 and 65 % of nominal. Therefore the toxicological endpoints were evaluated using nominal and actual concentrations of the test item.Toxicological endpoints
Endpoint
AD 1 [mg/L]
nominal
24 h
48h
NOEC
7.51
7.51
EC50
22.8
14.5
95 % confidence limit of EC50
18.1 – 28.6
11.7 – 17.9
Endpoint
AD 1 [mg/L]
actual*
24 h
48h
NOEC
5.26
5.26
EC50
14.7
9.28
95 % confidence limit of EC50
11.7 – 18.4
7.68 – 11.6
*based on the geometric mean measured content of fresh and aged samples of each concentration level
The total hardness (as CaCO3) of the untreated control was determined to be 13°dH (232 mg/L CaCO3); the mean pH-value of the untreated control was determined to be7.67±0.11 (Std. Dev.), the mean temperature of the control and all test item concentrations was measured to be 20.4±0.2 °C (Std. Dev.) and the mean oxygen concentration was determined to be 8.6±0.5 mg/L (Std. Dev.).
Reference
Results of the test, 24 h values
Concentration Test Item |
Control |
3.42 |
7.51 |
16.5 |
36.4 |
80.0 |
|
[mg/L] |
|||||
|
immobilised daphnids after 24 h |
|||||
Group 1 |
0 |
0 |
0 |
3 |
3 |
5 |
Group 2 |
0 |
0 |
0 |
3 |
3 |
5 |
Group 3 |
0 |
0 |
0 |
0 |
4 |
5 |
Group 4 |
0 |
0 |
0 |
1 |
5 |
5 |
Σ |
0 |
0 |
0 |
7 |
15 |
20 |
% |
0 |
0 |
0 |
35 |
75 |
100 |
Results of the test, 48 h values
Concentration Test Item |
Control |
3.42 |
7.51 |
16.5 |
36.4 |
80.0 |
|
[mg/L] |
|||||
|
immobilised daphnids after 48 h |
|||||
Group 1 |
0 |
0 |
0 |
3 |
5 |
5 |
Group 2 |
0 |
0 |
0 |
3 |
5 |
5 |
Group 3 |
0 |
0 |
2 |
3 |
5 |
5 |
Group 4 |
0 |
0 |
0 |
2 |
5 |
5 |
Σ |
0 |
0 |
2 |
11 |
20 |
20 |
% |
0 |
0 |
10 |
55 |
100 |
100 |
Results of the toxic reference, started on 10 Nov 2015
|
24 h |
48 h |
||||
K2Cr2O7 |
1.0 |
2.0 |
1.0 |
2.0 |
||
[mg/L] |
immobilised daphnids |
|||||
Group 1 |
0 |
1 |
3 |
5 |
||
Group 2 |
0 |
5 |
1 |
5 |
||
Group 3 |
0 |
5 |
0 |
5 |
||
Group 4 |
0 |
3 |
4 |
5 |
||
S |
0 |
14 |
8 |
20 |
||
% |
0 |
70 |
40 |
100 |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 9.28 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.