Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 911-418-6 | CAS number: 55965-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Test material form:
- other: Amber liquid
- Details on test material:
- - Name of test material (as cited in study report):Kathon(TM) 886F biocide
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rohm and Haas, Batch No. 6111L019
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable at room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Soluble and stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dilution in water
- Final dilution of a dissolved solid, stock liquid or gel: 1, 10, 50, 100 and 500 mg/L
OTHER SPECIFICS: Purity of test material was 14.13%
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): Laboratory mixer
- Controls: Negative control
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm (Eisenia foetida andrei)
- Source: Blades Biological, Kent
- Age at test initiation (mean and range, SD): 2 months
- Weight at test initiation (mean and range, SD): 300-600 mg
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): acclimated in artificial soil for at least 7 days prior to study initiation
- Health during acclimation (any mortality observed): Not described
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 20 - 21 °C
- pH:
- 6.8-7.0
- Moisture:
- Approximately 35 %
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): Glass beakers of 1 liter capacity covered with perforated plastic film
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): 20 % kaolinite clay, 70 % sand, 8 % sphagnum peat moss
OTHER TEST CONDITIONS
- Photoperiod: Not described
- Light intensity: 608 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Worms: daily observations, mortality (days 7 and 14), live weights (days 0 and 14).
Soil: moisture content, pH
Environmental area: temperature
VEHICLE CONTROL PERFORMED: not applicable - Nominal and measured concentrations:
- Nominal: 0, 62.5, 125, 250, 500 and 1000 mg whole product /kg dry weight soil
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 8.8 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 86.6 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.71 mg/kg soil dw
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 6.96 mg/kg soil dw
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: No mortality observed in control, 87.5 % at the highest concentration after 14d. Other treatments showed 17.5% or lower mortality.
- Total mass of adults at beginning of test: Not described
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: Kathon™ 886F had no significant effect on earthworm weight under the conditions of this test.
- No. of offspring produced: Not described
- No. of unhatched cocoons: Not described
- Morphological abnormalities: Not described
- Behavioural abnormalities: No
- Other biological observations: Earthworms at 1000 and 500 mg/kg were observed on the soil surface from test commencement through day 10. - Reported statistics and error estimates:
- The median lethal concentration (LC50) at 7 and 14 days was determined by probit analysis (Finney D.J., 1971, Probit analysis, 3rd edition, Cambridge University Press). Differences in body weight were analyzed by analysis of variance (ANOVA). Pairwise comparisons between each of the doses and the control were performed using Dunnett’s test at the 5 % significance level. The probit transformed concentrations of TS were subject to a regression procedure against logarithmically transformed concentrations of TS with a Newton-Raphson maximum likelihood iterative procedure being used to obtain parameter estimates. From the model the LC50 value was estimated. The goodness of fit was checked via the Pearson chi-squared test statistic. A significant chi-squared test statistic indicated heterogeneity of variances between the observed and expected % mortality values. If the test statistic was significant at the 1 % level, the variances and covariances were adjusted by a heterogeneity factor. Following this, the confidence limits were not reported and the LC50 values should be treated with caution.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Mortality of control animals < 10 %
- Conclusions:
- 14d LC50 (survival, TWA) = 6.96 mg a.i. /kg dry soil ; 14d LC50 (survival, nominal) = 86.6 mg a.i. /kg dry soil
14d NOEC (survival, TWA) = 0.71 mg a.i. /kg dry soil; 14d NOEC (survival, nominal) = 8.83 mg a.i. /kg dry soil - Executive summary:
OECD Method 207, Acute toxicity to the earthworm. Kathon™886F had no significant effect on earthworm weight under the conditions of this test. KathonTM 886F is not highly toxic to earthworms over a 14-day test period. 14 dLC50= 613 mg/kg whole product (soil); 86.6 mg a.i./kg (soil); 14 d NOEC = 62.5 mg whole product/kg (survival); 8.8 mg a.i./kg.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Although ECHA is providing a lot of online material in your language, part of this page is only in English. More about ECHA’s multilingual practice.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
the-echa-website-uses-cookies
find-out-more-on how-we-use-cookies