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EC number: 235-546-0 | CAS number: 12270-13-2
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: screening test, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For justification of read-across please refer to the read-across report attached to IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Remarks on result:
- other: for results refer to the section BOD5/COD results
- Key result
- Parameter:
- BOD5
- Value:
- 4 mg O2/g test mat.
- Key result
- Parameter:
- COD
- Value:
- 1 384 mg O2/g test mat.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable.
- Executive summary:
The chemical oxygen demand (COD) of the test substance was investigated in a test according to method EU C.9.
2.5 mg of the test substance were chemically oxidised with potassium dichromate at 148 +/- 3 °C for 2h. The amount of unreacted potassium dichromate is determined by titration and the COD is calculated from this value. The COD of the test substance was determined to be 1384 mg O2/g under the conditions of this test.
The biological oxygen demand after 5 days (BOD5) of the test substance was investigated in a test according to ISO 5815 second edition 1989-08-01 (E).
The test substance was incubated with seeding water of an urban waste water at concetrations ranging between 806.9 and 6.3 mg/L for 5 days at 20 +/- 1 °C without supression of nitrification. Oyxgen measurements were conducted with an oxygen sensitive electrode (SYLAND).
The biological oxygen demand after 5 days (BOD5) of the test substance is 4 mg O2/g.
The COD is a measure for the amount of organic compounds which can be chemically oxidised. The BOD5 provides information on the amount of biodegradable organic matter. As the BOD5/COD-ratio of the test substance is 0.003, the test substance can be considered not readily biodegradable.
This information is used in a read-across approach in the assessment of the target substance.
For justification of read-across please refer to the attached read-across report (see IUCLID section 13).
Reference
Description of key information
The chemical oxygen demand (COD) of the test substance was investigated in a test according to method EU C.9 and was determined to be 1384 mg O2/g (Kronenberg, 1991).
Measurements of the biological oxygen demand after 5 days (BOD5) of the test substance were conducted according to ISO 5815, second edition 1989-08-01 (E), and resulted in a value of 4 mg O2/g (Kronenberg, 1992).
The COD is a measure for the amount of organic compounds which can be chemically oxidised. The BOD5 provides information on the amount of biodegradable organic matter.
The BOD5 test is a traditional biodegradation test that is now replaced by the ready biodegradability tests and which should therefore not be performed today for assessment of the ready biodegradability of substances. However, only inherent biodegradability data are available (Dietschy, 1992). Hence, the BOD5/COD measurements will be used as main biodegradability indicator.
If substantial degradation occurs within a short period, the situation may be compared with the criterion BOD5/COD ≥ 0.5. In this case, a compound can be considered readily degradable (and hence rapidly degradable), if:
• the ultimate biodegradability exceeds 50% within 5 days and
• the ultimate degradation rate constant in the test system in this period is greater than 0.1 day-1 corresponding to a half -life of 7 days in the test system.
Based on the OECD 302B test (Dietschy, 1992), it can be concluded that the test item is not inherently biodegradable but strongly adsorbs to activated sludge particles. As the BOD5/COD-ratio of the test substance is 0.003, the test substance can be considered not readily/rapidly biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
This information is used in a read-across approach in the assessment of the target substance.
For justification of read-across please refer to the attached read-across report (see IUCLID section 13).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
