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EC number: 807-112-6 | CAS number: 1393708-17-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18.11.2014 to 20.11.2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- (2004)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- (2008)
- Qualifier:
- according to guideline
- Guideline:
- ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
- Version / remarks:
- (2010)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- LAUS GmbH, Auf der Schafweide 20, D-67489 Kirrweiler (Germany)
Test material
- Reference substance name:
- 3-(diethylcarbamoyl)-1-(3-sulfonatopropyl)pyridin-1-ium
- EC Number:
- 807-112-6
- Cas Number:
- 1393708-17-2
- Molecular formula:
- C13H20N2O4S
- IUPAC Name:
- 3-(diethylcarbamoyl)-1-(3-sulfonatopropyl)pyridin-1-ium
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): Pyridiniumsulfonate
- Physical state: white powder
- Analytical purity: 99.8% (HPLC)
- Impurities (identity and concentrations):
- Purity test date:
- Lot/batch No.: CHZT000001
- Expiration date of the lot/batch: 2/2016
- Stability under test conditions: stable
- Storage condition of test material: test material stored in closed vessel dark and dry at room temperature (min. -5 - max. +40°C)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The content of the test item in the test vessels was determined at the beginning and at the end of the test via HPLC/UV
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea (Daphnia magna Straus)
- Strain: Berlin
- Source: Umweltbundesamt Berlin
- Age at study initiation (mean and range, SD): <=24 h
- Method of breeding: Daphnia magna is bred in the LAUS GmbH throughout the year. The animals multiply by parthenogenesis. The husbandry is performed similar to the method described in EN ISO 6341: vessels: preserving glasses (nominal volume: 2 L), medium: M4-Medium, medium renewal: twice a week, food: unicellular green algae (Desmodesmus subspicatus), photo period: 16/8 h using neon tubes, temperature: 20 +/- 2°C
- Feeding during test: no
ACCLIMATION
- Acclimation period: not necessary
- Acclimation conditions (same as test or not): Switching from M4-medium (husbandry) to ISO medium (test) had been shown not to cause any detrimental effects for test Daphnia
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- dilution water: 250 mg CaCO3/L
- Test temperature:
- 19.2-19.8°C
- pH:
- pH value in the test media and the control at time 0 and after 48 h: 7.8
- Dissolved oxygen:
- dissolved oxygen in the test media and the control at time 0 and after 48 h: 8.5-8.7 mg/L
- Nominal and measured concentrations:
- nominal: 100 mg/L
measured
a. t=0 h: 100.84 mg/L (corresponding to 101% of nominal)
b. t=48 h: 105.64 mg/L (corresponding to 105.64% of nominal) - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beaker
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass/50 mL/-/20 mL
- Aeration: aeration of dilution water
- Type of flow-through (e.g. peristaltic or proportional diluter): test conducted under static conditions
- Renewal rate of test solution (frequency/flow rate): test conducted under static conditions
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): no vehicle used
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: yes, switching from M4-medium (husbandry) to ISO medium (test) had been shown not to cause any detrimental effects for test Daphnia
- Intervals of water quality measurement: pH and dissolved oxygen were measured at the beginning and at the end of the test
OTHER TEST CONDITIONS
- Adjustment of pH: pH of dilution water was adjusted to pH 7.8 +/-0.2
- Photoperiod: diffuse lighting
- Light intensity: not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
- Justification for using less concentrations than requested by guideline: limit test
- Range finding study: not reported - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Potassium dichromate was used as positive control in a current reference study which was performed in August 2014. The 24 h EC50 was determined as 1.2 mg/L, lying within the demanded range of 0.6-1.7 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a limit test conducted according to OECD Guideline 202, EU Method C.2, and ISO 6341 15, the 48 h EC50 and NOEC were determined to be >100 mg/L and >=100 mg/L (nominal each), respectively.
- Executive summary:
The acute toxicity of pyridiniumsulfonate towards Daphnia magna was investigated in a study conducted according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test), EU Method C.2 (Acute Toxicity for Daphnia; 2008), and ISO 6341 15 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea) 2010). 20 daphnids (5 daphnids/replicate) were exposed to pyridiniumsulfonate for 48 h at a nominal concentration of 100 mg/L under static conditions. The test material concentration was determined by HPLC/UV measurements. The 48 h EC50 and NOEC were determined to be >100 mg/L and >=100 mg/L (nominal each), respectively.
The study is considered as reliable without restriction and fulfills the requirements of the guideline.
Results Synopsis
Test organism: Daphnia magna Straus (<=24 h old). Concentrations in nominal values, analytically verified.
Test Type: static
48 h EC50: >100 mg/L48 h NOEC>=100 mg/L
Endpoint(s) Effected: mobility
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