2H-Tetrazol-5-amine, potassium salt (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

yes

Remarks:

Only one of the two apparatus blanks could be evaluated due to a contamination in the flask and Samples of day 23 could not be included in the evaluation due to an instrumental failure in the carbon analyser. Both doesn’t affect the outcome of the study.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

potassium 5-amino-1H-1,2,3,4-tetrazol-1-ide

Cas Number:

136369-04-5

Molecular formula:

CH2KN5

IUPAC Name:

potassium 5-amino-1H-1,2,3,4-tetrazol-1-ide

Test material form:

solid: particulate/powder

Details on test material:

Batch no.: 08KI030

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic (adaptation not specified)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

205.2 mg/L

Based on:

test mat.

Remarks:

nominally

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

other: Phenylamine (CAS# 62-53-3)

Results and discussion

% Degradation

Key result

Parameter:

% degradation (CO2 evolution)

Value:

4

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

All validity criteria were met. Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation was not observed. Both replicates of the test item showed very good correspondence. If degradation in the toxicity flask is below 25% after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 37% after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 206.6 mg/L”. The result of the test can be considered valid. The test item 5-Aminotetrazol Potassium is considered as “not readily biodegradable“. The degree of biodegradation reached 4 % after 28 days. No 10-day-window could be identified and the pass level of 60 % given in the OECD guideline was missed. No abiotic degradation was observed.

Executive summary:

The test item 5-Aminotetrazol Potassium was tested using a concentration of nominally 20 mg organic carbon/L (corresponding to 205.2 mg 5-Aminotetrazol Potassium/L) in test

medium following OECD 301B and EU-Method C.4-C. Aniline was chosen as positive control. Activated sludge was used as inoculum (concentration in the test 25.1 mg dry matter/L). The test was left running for 28 days. All validity criteria were met. Degradation of the positive control was 75 % after nine days. The following data could be determined for the test item 5-Aminotetrazol Potassium:

10-day-window:                                          not detected

degradation at the end of 10-day-window:    none

degradation at the end of the test                  4 %

pass level:                                                  60% at the end of 10-day-window

Therefore, regardless of the 10-day-window, 5-Aminotetrazol Potassium is not readily biodegradable following OECD 301B/EU C.4-C.