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EC number: 239-590-1 | CAS number: 15541-60-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study conducted in accordance with an internationally recognised method
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: a preliminary study had identified the 72 hour EC50 as being approximately 39mg/l by Biomass integral and therefore the definitive test concentrations were prepared as Control (0) 10. 18. 32, 56 and 100mg
- Sampling method: the samples are transferred to 8m1 polypropylene vials and analysed by Ion Chromatography.
- Sample storage conditions before analysis: test substance specific analysis of each test concentration was carried out as soon as possible after sampling. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a 100 mg/1 solution of the sample was prepared in culture medium. A clear colourless solution was obtained. The test concentrations prepared by addition of the appropriate volume of this solution to the algal culture medium. The definitive test concentrations
were prepared as Control (0) I 0, 18, 32, 56 and 100 mg/1.
Note: after 72 hours incubation the test solutions were observed to be faintly green and cloudy at 100 mg/1 due to the low cell density. At lower concentrations the exposure solutions had a strong green colouration and turbidity due to the high algal cell density. There was no sedimented
material apparent.
- Controls: as for test solution but without addition of test substance
-
Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable, none used
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 100 mg/L stock.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): there was no sedimented material apparent. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CCAP 278/4
- Source: culture collection
- Method of cultivation: from a pre-culture growing in exponential phase. Inoculum level adjusted to give an initial cell density of 1 x 10E4
cells/ml. Culture conditions same as test conditions (ca. 6000 to 10000 lux continuous white light, temperature 23 ± 2°C, shaking at 200 rpm). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- Not reported
- Test temperature:
- 23 ± 2 °C
- pH:
- 0 mg/l 7.8 (start) 8.4 (end)
10 mg/l 7.4 (start) 8.4 (end)
18 mg/l 7.1 (start) 7.5 (end)
32 mg/l 7.0 (start) 6.9 (end)
56 mg/l 6.4 (start) 6.5 (end)
100 mg/l 5.9 (start) 5.9 (end) - Dissolved oxygen:
- not reported
- Salinity:
- not applicable - freshwater study
- Nominal and measured concentrations:
- Nominal: 0, 10, 18, 32, 56 and 100mg/I.
Measured: -, 14%, 42%, 53%, 83% and 94% - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml conical flask
- Fill volume: 100 ml
- Aeration: no
- Initial cells density: 1 x 10E4 cells/ml
- Control end cells density: 202 x 10E4 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: de-ionised, filtered and sterilised by autoclaving at 120°C for 30 minutes.
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: adjusted to 8.0 ± 0.2 to obtain the culture medium.
- Photoperiod: continuous
- Light intensity and quality: 6000 - 10000 lux white light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: the cell density was determined at 24, 48 and 72 hours on a small volume removed from each replicate flask. The cell counts were made using a haemocytometer and microscope.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: yes
- Test concentrations: Control (0), 10, 18, 32, 56 and 100 mg/1.
- Results used to determine the conditions for the definitive study: A preliminary study identified the 72 hour EC50 as being approximately 39mg/l by Biomass integral. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 44 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 98 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
The water quality measurements of the test solutions (pH and temperature) were within acceptable limits.- Results with reference substance (positive control):
- - Results with reference substance valid?
- EC50:
- Other: - Reported statistics and error estimates:
- NOEC estimated as <10 mg/1 as determined by the Bonferroni T test, (P = 0.05)
- Validity criteria fulfilled:
- yes
- Remarks:
- pH and temperature of test medium within acceptable limits. Cell density of controls increases by a factor of at least 16 within 72hrs.
- Conclusions:
- The 72 hour EC50 by growth rate was determined to be 98 mg/l.
Reference
Percent inhibition by biomass integral and growth rate
Nominal concentration (mg/l) |
Percent inhibition by biomass integral |
Percent inhibition by growth rate |
||
0-48hours |
0-72hours |
0-48hours |
0-72hours |
|
10 |
8 |
21 |
6 |
6 |
18 |
15 |
18 |
7 |
4 |
32 |
IS |
27 |
4 |
8 |
56 |
26 |
66 |
14 |
32 |
100 |
56 |
83 |
30 |
50 |
The 72 hour EC50 of MPP to Pseudokirclmeriella subcapitata was estimated as 44mg/l using the biomass integral and 98mg/l by growth rate calculation, nominal concentrations. The 48 hour EC50 was 93mg/l by biomass integral and > 1OOmg/1 by growth rate, nominal concentrations. The 72 hour NOEC was estimated as < 10 mg/1 as determined by the Bonferroni T test.
The growth curves showed that at test concentrations 10 to 32 mg/l, the growth of the algae was good with a low level of inhibition at 48 hours, increasing a little at 72 hours. At higher concentrations of 56 to l00 mg/1 a clearer dose response was recorded with very little increase in cell density between 48 and 72 hours.
Description of key information
The 72 hour EC50 by growth rate was determined to be 98 mg/l.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 98 mg/L
Additional information
In a test for algal growth inhibition conducted over 72 hours, the growth curves showed that at test concentrations 10 to 32 mg/l, the growth of the algae was good with a low level of inhibition at 48 hours, increasing a little at 72 hours. At higher concentrations of 56 to l00 mg/1 a clearer dose response was recorded with very little increase in cell density between 48 and 72 hours.
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