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EC number: 253-197-2 | CAS number: 36768-62-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study, without detailed documentation
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1981
- Principles of method if other than guideline:
- acc. to Ames et al. 1975
- GLP compliance:
- no
- Remarks:
- prior to GLP settings
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2,6,6-tetramethyl-4-piperidylamine
- EC Number:
- 253-197-2
- EC Name:
- 2,2,6,6-tetramethyl-4-piperidylamine
- Cas Number:
- 36768-62-4
- Molecular formula:
- C9H20N2
- IUPAC Name:
- 2,2,6,6-tetramethylpiperidin-4-amine
- Details on test material:
- no data
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix from Arochlor induced rat liver
- Test concentrations with justification for top dose:
- 10, 100, 500, 1000 ug/pl.
- Vehicle / solvent:
- water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- Positive controls:
- no
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- Migrated to IUCLID6: TA 100 in DMSO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation; plate with agar
DURATION
- Preincubation period: 30 min
NUMBER OF REPLICATIONS: 2
DETERMINATION OF CYTOTOXICITY
not explicit reported, but from the number of revertants, no cytotoxicity was observed
- Evaluation criteria:
- According to Ames et al. 1975
Substances, which showed no effects even with 500 µg/plate, no mutagenic effects are expected. To give higher safety, the highest concentration chosen was 1000 µg/plate - Statistics:
- none
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not examined
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Values presented as mean of mutants per plate (three replicates) and standard deviation in brackets
with S9 mix
strain /conentration [µg/plate] | control (water) | 10 | 100 | 500 | 1000 |
TA 98 | 29 (7.7) | 24 (2) | 28 (6.6) | 31 (1.1) | 28 (9.2) |
TA 100 | 31 (9.5) | 25 (7.5) | 34 (5.1) | 36 (2.6) | 35 (2.6) |
TA 1535 | 12 (2.3) | 11 (4.1) | 10 (2.5) | 13 (5.6) | 12 (3.6) |
TA 1537 | 11 (3.5) | 6 (2.9) | 11 (1.7) | 9 (4.3) | 11 (7) |
TA 1538 | 27 (8.6) | 27 (5.5) | 26 (1.5) | 27 (1) | 27 (7.5) |
without S9 mix
strain /conentration [µg/plate] | control (water) | 10 | 100 | 500 | 1000 |
TA 98 | 16 (3.2) | 15 (5.1) | 16 (2.8) | 15 (1.1) | 20 (4.7) |
TA 100 | 37 (10.5) | 44 (19.5) | 39 (10.4) | 37 (0.5) | 34 (6.2) |
TA 1535 | 11 (2.5) | 9 (2.8) | 12 (2.6) | 6 (4.3) | 8 (3.6) |
TA 1537 | 11 (3.4) | 9 (2.5) | 9 (1.5) | 12 (1.9) | 8 (1.7) |
TA 1538 | 15 (6.6) | 16 (4.1) | 13 (3.6) | 15 (5.5) | 12 (3) |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
Under the present conditions, no mutagenic effects in all tested strains were observed in the bacterial gene mutation assay up to concentrations of 1000 µg/plate.
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