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Diss Factsheets
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EC number: 204-679-6 | CAS number: 124-09-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: dermal
Administrative data
- Endpoint:
- repeated dose toxicity: dermal
- Remarks:
- other: no data
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- no data
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: The document is insufficient for assessment (protocol, test animal, test material, parameters examined......)
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 937
- Report date:
- 1937
Materials and methods
Test guideline
- Qualifier:
- no guideline required
- Principles of method if other than guideline:
- The aim of this study was to test the toxicity of Hexamethylene Diamine in white rats, by application to the skin.
Six white rats were treated daily on 5 days of the week on their shaved backs with a 1% paste of crude hexamethylene diamine in vaseline. No precautions were taken to prevent licking. Animals were killed on the twenty-first day of the experiment, after 16 applications.
In a second series of experiments, 6 rats were similarly treated with a 2% paste of crude HMD. Rats were killed on the ninth day of the experiment, after 7 applications of test substance. - GLP compliance:
- no
- Remarks:
- Study performed before GPL establishment
- Limit test:
- yes
Test material
- Reference substance name:
- Hexamethylenediamine
- EC Number:
- 204-679-6
- EC Name:
- Hexamethylenediamine
- Cas Number:
- 124-09-4
- Molecular formula:
- C6H16N2
- IUPAC Name:
- hexane-1,6-diamine
- Details on test material:
- - Name of test material (as cited in study report): Hexamethylene Diamine (HMD)
- Substance type: no data
- Physical state: no data
- Analytical purity: no data
- Lot/batch No.: no data
- Storage condition of test material: no data
Constituent 1
Test animals
- Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- No data
Administration / exposure
- Type of coverage:
- open
- Vehicle:
- other: vaseline
- Details on exposure:
- TEST SITE
- Area of exposure: no data
- % coverage: no data
- Type of wrap if used: open
- Time intervals for shavings or clipplings: no data
REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data
- Time after start of exposure: no data
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1% paste of crude HMD in vaseline and 2% paste of crude HMD in vaseline
- Concentration (if solution): 1% and 2%
- Constant volume or concentration used: no data
- For solids, paste formed: no
VEHICLE
no data
USE OF RESTRAINERS FOR PREVENTING INGESTION: no - Analytical verification of doses or concentrations:
- not specified
- Details on analytical verification of doses or concentrations:
- No data
- Duration of treatment / exposure:
- First group: 16 applications
Second group: 7 applications - Frequency of treatment:
- Daily on 5 days of the week
- No. of animals per sex per dose:
- No data
- Details on study design:
- No data
- Positive control:
- no
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: No data
DETAILED CLINICAL OBSERVATIONS: No data
DERMAL IRRITATION (if dermal study): Yes
- Time schedule for examinations: no data
BODY WEIGHT: No data
FOOD CONSUMPTION: no data
FOOD EFFICIENCY: no data
WATER CONSUMPTION: No data
OPHTHALMOSCOPIC EXAMINATION: No data
HAEMATOLOGY: No data
CLINICAL CHEMISTRY: No data
URINALYSIS: No data
NEUROBEHAVIOURAL EXAMINATION: No data - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
HISTOPATHOLOGY: No data - Other examinations:
- No data
- Statistics:
- No data
Results and discussion
Results of examinations
- Clinical signs:
- not specified
- Dermal irritation:
- effects observed, treatment-related
- Mortality:
- not specified
- Body weight and weight changes:
- not specified
- Food consumption and compound intake (if feeding study):
- not specified
- Food efficiency:
- not specified
- Water consumption and compound intake (if drinking water study):
- not specified
- Ophthalmological findings:
- not specified
- Haematological findings:
- not specified
- Clinical biochemistry findings:
- not specified
- Urinalysis findings:
- not specified
- Behaviour (functional findings):
- not specified
- Organ weight findings including organ / body weight ratios:
- not specified
- Gross pathological findings:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- not specified
- Histopathological findings: neoplastic:
- not specified
- Details on results:
- No additional data
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
First group: the first few applications produced erythema and scaling of the skin, but these gradually dissapeared. At the end of the experiment, when animals were killed on the twenty-first day, new hair had grown almost completely over the shaved area. With the exception of mild degenerative changes in the liver cells in 3 out of 6 rats and mild to moderate regressive lesions of the renal tubules in 2 out of 6 animals, the pathological examination gave no evidence of any toxic effect in these animals.
Second group: rats showed sclaing of their epidermis and cracking of the skin developed after the third treatment and in five this lasted until they were killed on the ninth day of the experiment.
Upon autopsy, the treated area showed some sero-sanguinous crusts and liver and kidneys were mildly hyperemic.
Microscopically the skin showed small ulcerative defects of the epidermis, in other parts this was atrophic and contained small purulent. There were also inflammatory reactions in the lower layers of the skin. The only other pathological finding in these animals was a moderate degenerative in certain parts of the kidney.
Applicant's summary and conclusion
- Executive summary:
The aim of this study was to test the toxicity of Hexamethylene Diamine in white rats, by application to the skin. Six white rats were treated daily on 5 days of the week on their shaved backs with a 1% paste of crude hexamethylene diamine in vaseline. No precautions were taken to prevent licking. Animals were killed on the twenty-first day of the experiment, after 16 applications. In a second series of experiments, 6 rats were similarly treated with a 2% paste of crude HMD. Rats were killed on the ninth day of the experiment, after 7 applications of test substance.
With the first group, the first few applications produced erythema and scaling of the skin, but these gradually dissapeared. At the end of the experiment, when animals were killed on the twenty-first day, new hair had grown almost completely over the shaved area. With the exception of mild degenerative changes in the liver cells in 3 out of 6 rats and mild to moderate regressive lesions of the renal tubules in 2 out of 6 animals, the pathological examination gave no evidence of any toxic effect in these animals.
For the second group, rats showed sclaing of their epidermis and cracking of the skin developed after the third treatment and in five this lasted until they were killed on the ninth day of the experiment.
Upon autopsy, the treated area showed some sero-sanguinous crusts and liver and kidneys were mildly hyperemic.
Microscopically the skin showed small ulcerative defects of the epidermis, in other parts this was atrophic and contained small purulent. There were also inflammatory reactions in the lower layers of the skin. The only other pathological finding in these animals was a moderate degenerative in certain parts of the kidney.
These experiments indicated that Hexamethylene Diamine is strongly alkaline and extremely irritating. Systemic effects resulted in damage to the kidney.
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