Registration Dossier
Registration Dossier
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EC number: 415-430-8 | CAS number: 86403-32-9 CYASORB UV-3853 LIGHT STABILIZER; DASTIB 845; SANDUVOR 845
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- A mixture of: 2,2,6,6-tetramethylpiperidin-4-yl-hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl-octadecanoate
- EC Number:
- 415-430-8
- EC Name:
- A mixture of: 2,2,6,6-tetramethylpiperidin-4-yl-hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl-octadecanoate
- Cas Number:
- 86403-32-9
- Molecular formula:
- CnH2n+2O2, where n=12 to 18
- IUPAC Name:
- 2,2,6,6-tetramethylpiperidin-4-yl hexadecanoate; 2,2,6,6-tetramethylpiperidin-4-yl octadecanoate
Constituent 1
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain/clone:Straus
- Justification for species other than prescribed by test guideline:
- Source: laboratory own culture
- Age of parental stock (mean and range, SD): 24 h
- Feeding during test: yes
- Food type: Chlorococcal Alga
- Amount: 0.1 to 0.2 mg/L TOC
- Frequency:1/day
ACCLIMATION
- Acclimation period:4 week
- Acclimation conditions (same as test or not):not
- Type and amount of food:Chlorococcal Alga
- Feeding frequency:1/day
- Health during acclimation (any mortality observed): no
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- > 140 mg/L
- Test temperature:
- 20 °C ± 2°C, constant in this range ±1°C
- pH:
- 6-9, it should not change by more than 1.5 during test
- Dissolved oxygen:
- > 3 mg/L, withouth aeration
- Nominal and measured concentrations:
- nominal: 0.054 , 0.081, 0.12, 0.18, 0.27, mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:glass vessels
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 150 ml
- Aeration:no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates):10
- No. of vessels per vehicle control (replicates):0
OTHER TEST CONDITIONS
- Adjustment of pH: yes, at the start and in the end
- Photoperiod:16 h light/ 8h dark
- Light intensity: 600 - 800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, , number of living juveniles, pH, dissolved oxygen conc., temperature, light intensity, water hardness, TOC, test substance stability control
VEHICLE CONTROL PERFORMED: no
RANGE-FINDING STUDY
- Test concentrations:0.054 -0.27 mg/L - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 %CL
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.054 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.081 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality of parent animals: 10%
- No. of offspring produced per day per female:
- Number of males and females (parental): no males
- Time to first brood release or time to hatch: 6th day
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- As already mentioned above, all test conditions were fulfilled during the performed water flea Daphnia magna reproduction study.
10% mortality of parential females of water flea Daphnia magna in the control group was recorded. The first offspring in the control appeared as soon as on day 6 (i.e.before day 11), the mean number of surviving juveniles per parent organism in the control was 138. Variation coefficient of the number of surviving juveniles per parent individual in the control=10.9% and therefore the results of the water flea Daphnia magna reproduction toxicity test are considered valid. According to the results of the water flea Daphnia magna reproduction toxicity test the following value were determined for the test substance DASTIB 845:
21d EC50= 0.19 mg/L
NOEC = 0.054 mg/L
LOEC = 0.081 mg/L
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