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Diss Factsheets
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EC number: 260-828-5 | CAS number: 57583-34-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- not applicable
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: mixtures contained 16.0 mL of synthetic sewage, 200 mL of inoculum, the appropriate amount of test substance and enough municipal water to bring the total volume up to 500 mL. All mixtures were aerated and stirred for three hours.
- Controls: two controls were prepared
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Cambridge Wastewater Treatment Plant, Cambridge, Maryland
- Preparation of inoculum for exposure: The sludge was sieved using a 2 mm screen and allowed to settle for approximately 30 minutes. The supernatant above the settled solids was removed and the total suspended solids (TSS) concentration of the settled sludge was determined. Total suspended solids in the settled sludge were adjusted to a nominal concentration of approximately 4000 mg/L by dilution with municipal water. 50 mL of synthetic sewage was added to each liter of adjusted sludge. The sludge was maintained at a temperature of 21 ± 1°C and continuously aerated overnight.
The initial total suspended solids (TSS) concentration of the activated sludge after removal of the supernatant layer measured 17 300 mg/L. The measured TSS concentration of the sludge on the day of testing was 4347 mg/L. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- not applicable
- Hardness:
- no data
- Test temperature:
- 20.1 - 21.3 °C.
- pH:
- sludge- 7.3
- Dissolved oxygen:
- used to calculate respiration rates between 6.5 mg O2/L and 2.5 mg O2/L,
- Salinity:
- no data
- Nominal and measured concentrations:
- 10, 30, 100, 300 and 1000 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: mixtures were prepared, mixed and aerated in 1000 mL Erlenmeyer flasks and then transferred to fill 300 mL biochemical oxygen demand (BOD) bottles to conduct dissolved oxygen (DO) measurements.
- Aeration: aerated for three hours
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates): one/concentration
- No. of vessels per control (replicates): duplicate
- No. of vessels per vehicle control (replicates): not applicable
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal
- Culture medium different from test medium: no
- Intervals of water quality measurement: no data. Direct reading of dissolved oxygen concentration using a using a YSI Model 50B-115 Dissolved Oxygen Meter
OTHER TEST CONDITIONS
- Adjustment of pH: none
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : dissolved oxygen concentrations were measured every 10 seconds over a 15 minute period. Respiration rates and percent inhibitions were calculated from this.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no
- Substance was tested at far above water solubility limit, which is estimated to be 4.89 x 10E-7 mg/L. For this reason, direct addition of the test substance (by weight), and 3 hour contact time were used. Stimulation of respiration (6.1 - 181 % ) was observed over 4 of the 5 tested concentrations (11 % inhibition was observed for the other concentration), indicating that the test substance was being used as a substrate for growth/energy by the activated sludge microorganisms. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at concentrations of 3, 15 and 50 mg/l. The respiration rates for two controls were 45.3 and 40.1 mg O2/l/hr. The difference between the two control respiration rates was approximately 12 % and was within the 15 % difference limit established for the test. The validity of the test was supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 of 11.7 mg/l and was within the 5 to 30 mg/L range considered acceptable for the test. - Reported statistics and error estimates:
- When the dose response pattern (percent inhibition versus test substance concentration) allowed for the calculation of an EC50 value, the data were analysed using the computer program of C.E. Stephan. The program was designed to calculate the EC50 value and the 95% confidence interval by probit analysis, the moving average, or binomial probability with nonlinear interpolation.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest percent inhibition value obtained in the study, for the test substance vessels, was 11.0 %. The EC50 value for the test substance in this study is therefore greater than 1000 mg/L, the highest concentration tested.
- Executive summary:
The effect of the test substance on activated sludge microorganisms maintained in an aerobic environment was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline 209). The test contained control, reference and treatment groups. The control replicates were used to determine the background respiration rate of the sludge and were not dosed with the test or reference substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at concentrations of 3, 15 and 50 mg/L. The treatment group was dosed with test substance at concentrations of 10, 30, 100, 300 and 1000 mg/L. After an exposure period of three hours, the respiration rates of the test solutions were measured using a YSI Model 50B-115 Dissolved Oxygen Meter. The respiration rates in the two controls were 45.3 and 40.1 mg O2/L/hr. The difference between the two control respiration rates was approximately 12 %, and was within the 15 % difference limit established for the test. The validity of the test was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 of 11.7 mg/L. The EC50 for the reference substance was within the 5 to 30 mg/L range considered acceptable for the test. The highest percent inhibition value obtained in the study, for the test substance vessels, was 11.0 %. The EC50 value is therefore greater than 1000 mg/L, the highest concentration tested.
Reference
Description of key information
Activate sludge EC50 >1000 mg/L (OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The effect of the test substance on activated sludge microorganisms maintained in an aerobic environment was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline 209). The test contained control, reference and treatment groups. The control replicates were used to determine the background respiration rate of the sludge and were not dosed with the test or reference substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor of respiration, at concentrations of 3, 15 and 50 mg/L. The treatment group was dosed with test substance at concentrations of 10, 30, 100, 300 and 1000 mg/L. After an exposure period of three hours, the respiration rates of the test solutions were measured using a YSI Model 50B-115 Dissolved Oxygen Meter. The respiration rates in the two controls were 45.3 and 40.1 mg O2/L/hr. The difference between the two control respiration rates was approximately 12 %, and was within the 15 % difference limit established for the test. The validity of the test was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 of 11.7 mg/L. The EC50 for the reference substance was within the 5 to 30 mg/L range considered acceptable for the test. The highest percent inhibition value obtained in the study, for the test substance vessels, was 11.0%. The EC50 value is therefore greater than 1000 mg/L, the highest concentration tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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