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Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
Highest test concentration : 1000 mg/l
Lowest test concentration : 500 mg/l

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Preparation of inoculum for exposure: The concentrated inoculum suspension was aerated over 1 day. Nutrition solution was added during this period, as mentioned in the test guideline.
- Pretreatment: corresponding to the cited test guideline.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
The incubation temperature was controlled at 20.8°C to 21.8°C.
pH:
6.3-8.2 after the incubation of the test batches.
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
growth inhibition
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
growth inhibition
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
growth inhibition
Details on results:
Testing the respiration inhibition of activated sludge proceeded without disturbances.
Results with reference substance (positive control):
The EC50 of the reference substance 3,5-Dichlorophenol was between 5 mg/l and 30 mg/l and amounted to 16 mg/l. The range of confidence could not be determined. Raw data were calculated with Probit Analysis according to Finney (1971).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A significant inhibition could not be observed. The documented EC-values were above the highest tested concentration.