Ethyl 3-[[bis(1-methylethoxy)phosphinothioyl]thio]propionate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

non GLP, no bw developement, no necropsy, considered of minor importance due to absence of mortality and overt signs of toxicity

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

A pilot acute oral toxicity study carried out on groups of 4 young adult rats (2 male, 2 female) at dose levels of 2.5, 5 and 10 ml/kg, followed by an acute oral toxicity study carried out on a group of 10 young adult rats (5 female, 5 male) at a dose level of 5 ml/kg. The rats were weighed in groups of 2 or 5 and the average bodyweight used to calculate the volume administered.

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 3-[[bis(1-methylethoxy)phosphinothioyl]thio]propionate

EC Number:

275-965-6

EC Name:

Ethyl 3-[[bis(1-methylethoxy)phosphinothioyl]thio]propionate

Cas Number:

71735-74-5

Molecular formula:

C11H23O4PS2

IUPAC Name:

ethyl 3-{[bis(propan-2-yloxy)(sulfanylidene)-λ⁵-phosphanyl]sulfanyl}propanoate

Test animals

Species:

rat

Strain:

other: Sprague-Dawley derived rats of the C.D. strain

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River (U.K. ) Limited, Margate, Kent, UK
- Weight at study initiation: 220-300g
- Housing: groups of 2 (pilot study) or 5 (main study) in plastic cages
- Diet: Oxoid Breeding Diet supplied by Herbert C. Styles (Bewdley) Limited, ad libitum
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 17 +/- 1
- Humidity (%): 50-70

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 5 ml/kg

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: A pilot acute oral toxicity study carried out on groups of 4 young adult rats (2 male, 2 female) at dose levels of 2.5, 5 and 10 mL/kg. 50% mortality was observed at the highest dose level (10 mL/kg) and no deaths at 2.5 and 5 ml/kg.

Doses:

2,5, 5 and 10ml/kg in the pre-test
5 mL/kg in the main test

No. of animals per sex per dose:

2 in the pre-test
5 in the main test

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Other examinations performed: The animals were observed for signs of toxicity at hourly intervals for the 8 hours following treatment and daily for the subsequent 14 days.

Results and discussion

Preliminary study:

In the pilot study 50% mortality was observed at the highest dose level (10 mL/kg). No deaths occurred in rats treated at 2.5 and 5mL/kg.

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 mL/kg bw

Mortality:

50% at 10 mL /kg in the pilot study.

Clinical signs:

other: The two animals dying in the pilot study were lethargic and adopted a hunched stance 24 hours after treatment which persisted until death. No overt signs of toxicity w ere observed in the surviving animals.

Gross pathology:

not performed

Any other information on results incl. tables

Dose Level (mL/kg)	Animals	Average weight (g)	Volume dosed (mL)	Number surviving
Pilot study				day	1	2	3	4	5	6	7 -14
2.5	2 male	300	0.75		2	2	2	2	2	2	2
	2 female	250	0.63		2	2	2	2	2	2	2
5	2 male	250	1.25		2	2	2	2	2	2	2
	2 female	250	1.25		2	2	2	2	2	2	2
10	2 male	300	3		2	2	1	1	1	1	1
	2 female	220	2.2		2	1	1	1	1	1	1
Main study
5	5 male	250	1.25		5	5	5	5	5	5	5
	5 female	220	1.1		5	5	5	5	5	5	5

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The acute oral LD50 of the test substance is likely to be in excess of 5 ml/kg.

Executive summary:

An acute toxicity test on Sprague Dawley rats was performed to assess the toxic potential of the test substance after oral ingestion. In a pilot study carried out on groups of 4 young adult rats (2 male, 2 female) at dose levels of 2.5, 5 and 10 ml/kg, 50% mortality was observed at the highest dose level. No deaths occurred in rats treated at 2.5 and 5 ml/kg. The following main study was performed with a group of 10 young adult rats (5 female, 5 male) at a dose level of 5ml/kg, in which no mortalities were reported. Bases on this result, the test article is considered to be practically non toxic after a single oral ingestion.