Diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide

EC Number:

278-355-8

EC Name:

Diphenyl(2,4,6-trimethylbenzoyl)phosphine oxide

Cas Number:

75980-60-8

Molecular formula:

C22H21O2P

IUPAC Name:

(mesitylcarbonyl)(diphenyl)phosphine oxide

Details on test material:

- Name of test material (as cited in study report): Lucirin TPO
- Lot/batch No.: 0095, 0096
- Analytical purity: 97.3 %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added in the required amount to the test vessel with about 180 ml deionized water. The mixture was stirred for 22 ± 2 hours. Then the inoculum and synthetic medium was added and the test started.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Initial biomass concentration: 1 g/L dry substance

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

180 min

Test conditions

Test temperature:

20 +/- 2 °C

pH:

before correction: 6.7
after correction: 7.2

Nominal and measured concentrations:

Nominal test concentration (mg/l):
1000

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer vessels (nominal 250 mL)
- Fill volume: 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 100-fold concentrated OECD medium

Reference substance (positive control):

yes

Remarks:

3,5 dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

other: EC80

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate