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EC number: 203-807-8 | CAS number: 110-83-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 October, 2010
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 438(Isolated Chicken Eye Test Method for Identifying Ocular Corrosives and Severe Irritants)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 110-83-3
- IUPAC Name:
- 110-83-3
- Reference substance name:
- Cyclohexene
- EC Number:
- 203-807-8
- EC Name:
- Cyclohexene
- Cas Number:
- 110-83-8
- Molecular formula:
- C6H10
- IUPAC Name:
- cyclohexene
- Details on test material:
- Lot No. 10817K
Purity: 99.83%
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- other: chicken
Test system
- Vehicle:
- other: Sodium chloride (Salsol solution 0.9%)
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL
Amount(s) applied (volume with unit): 30μL - Duration of treatment / exposure:
- 10 seconds
- Observation period (in vivo):
- The control eye and test eyes were evaluated pre-treatment and at approximately 30, 60, 75, 120, 180 and 240 minutes after the post-treatment rinse. Minor variations within ±5 minutes were considered acceptable.
- Number of animals or in vitro replicates:
- N/A
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other: Maximum corneal swelling at up to 75min (%)
- Basis:
- mean
- Score:
- -2
- Remarks on result:
- other: ICE Class: Ⅰ
- Irritation parameter:
- other: Maximum corneal swelling at up to 240min (%)
- Basis:
- mean
- Score:
- -9
- Remarks on result:
- other: ICE Class: Ⅱ
- Irritation parameter:
- other: Maximum corneal opacity
- Basis:
- mean
- Score:
- 0.17
- Remarks on result:
- other: ICE Class: Ⅰ
- Irritation parameter:
- other: Fluorescein retention
- Basis:
- mean
- Score:
- 0.17
- Remarks on result:
- other: ICE Class: Ⅰ
- Irritation parameter:
- other: Other observation
- Remarks on result:
- other: None
- Irritation parameter:
- other: Overall ICE Class
- Remarks on result:
- other: 2×Ⅰ, 1×Ⅱ
Any other information on results incl. tables
RESULTS OF COTROL TEST
- Positive Control: Trichloroacetic acid 30 (w/v) %
Observation | Value | ICE Class |
Mean maximum corneal swelling at up to 75 min | -14% | Severe |
Mean maximum corneal swelling at up to 240 min | -21% | Severe |
Mean maximum corneal opacity | 3.83 | Ⅳ |
Mean fluorescein retention | 3.00 | Ⅳ |
Other Observations | Immediate cornea opacity score 4. Severe thinning (up to -21%) was observed in all eyes (3/3) at during the observation period. | |
Overall ICE Class | - | , 2×Ⅳ |
The positive control Trichloroacetic acid 30 (w/v) % was classed as severely irritating, GHS Classification: Category 1.
- Negative Control: Sodium chloride
Observation | Value | ICE Class |
Mean maximum corneal swelling at up to 75 min | 0% | Ⅰ |
Mean maximum corneal swelling at up to 240 min | 0% | Ⅰ |
Mean maximum corneal opacity | 0.00 | Ⅰ |
Mean fluorescein retention | 0.00 | Ⅰ |
Other Observations | None |
- |
Overall ICE Class | - | 3×Ⅰ |
The negative control Sodium chloride 0.9% had no significant effects on the chicken eye in this study.
※APPENDIX 1 TABLES OF ICE CLASSIFICATION
Table 2.1 ICE classification criteria for corneal class
Mean Corneal Swelling (%) | ICE Class |
0 to 5 | Ⅰ |
>5 to 12 † | Ⅱ |
>12 to 18 ( >75 min after treatment ) | Ⅱ |
>12 to 18 ( ≦75 min after treatment ) | Ⅲ |
>18 to 26 | Ⅲ |
>26 to 32 ( >75 min after treatment ) | Ⅲ |
>26 to 32 ( ≦75 min after treatment ) | Ⅳ |
>32 | Ⅳ |
† Negative numbers -5% to -12% are also given a grade II. Values below -12% are not classified.
Table 2.2 ICE classificartion for opacity
Mean Maximum Opacity Score | ICE Class |
0.0 – 0.5 | Ⅰ |
0.6 – 1.5 | Ⅱ |
1.6 – 2.5 | Ⅲ |
2.6 – 4.0 | Ⅳ |
Table 2.3 ICE classification criteria for mean fluorescein retention
Mean Fluorescein Retention Score at 30 minutes post - treatment | ICE Class |
0.0 – 0.5 | Ⅰ |
0.6 – 1.5 | Ⅱ |
1.6 – 2.5 | Ⅲ |
2.6 – 3.0 | Ⅳ |
※APPENDIX 2 CLASSIFICATION
Table 3.1 Assessment of the general IN VITRO eye irritancy and regulatory GHS classification.
The following table is used to identify the probably eye irritancy potential of test items. In the case where the result indicates Severely Irritating, then the test item can be classified as Severe. In all other cases the probable level of irritancy can be reported, but a regulatory in vivo rabbit eye irritation test is required for regulatory classification and labelling purposes.
Classification | Combinations of the three ICE Classes |
A = Not irritating | 3×I 2×I, 1×II 2xII, 1xI |
B = Slight irritating (GHS category 2B: Mild irritant / causes eye irritation) | 3×II 2×II, 1×III 1×I, 1×II, 1×III |
C = Moderate irritating (GHS category 2A: Irritant / causes eye irritation) | 3×III 2×III, 1×II 2xI, 1xIV 2×III, 1×IV 2×III, 1×I 2×II, 1×IV 1×II, 1×III, 1×IV |
D = Severely irritating (GHS category 1: Irreversible effects on the eye / serious damage to the eye) | 3×IV 2×IV, 1×III 2×IV, 1×II 2×IV, 1×I1 Immediate cornea opacity score 3 or 4 Severe loosening of epithelium |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information
- Conclusions:
- In vitro eye irritation study in the Isolated Chicken Eyes model with Cyclohexene, the results indicate that the test item was not irritating.
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