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EC number: 474-870-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 January 2007 to 06 February 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable. - Analytical monitoring:
- no
- Details on sampling:
- Test concentrations (mg/l) : 100, 1000 & 10000
** Test item concentration in physico-chemical oxygen consumption control (mg/l) : 10000 - Vehicle:
- no
- Details on test solutions:
- Type of application: direct weighing
Test concentration of the activated sludge: 960 mg/l suspended solids
Test temperature: 20 ± 2° C
Stirring period of test item before start of incubation time: 16 hours
Incubation time: 3 hours with permanent aeration - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Type: mixed population of aquatic microorganisms (activated sludge)
Origin: aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim)
Date of collection: 2007-01-29
Pretreatment: activated sludge, daily fed with synthetic medium
pH of the suspension before application: 7.8
Synthetic sewage feed:A synthetic sewage feed is made by dissolving the following amounts of substance in 1 litre of water:
16.0 g peptone
11.0 g meat extract
3.0 g urea
0.7 g NaCl
0.4 g CaCl2 - 2H20
0.2 g MgS04 – 7H20
2.8 g K2HP04
pH of the synthetic sewage feed: 7.5 ± 0.5 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- No data
- Test temperature:
- 18.8 - 19.5C
- pH:
- 7.1 - 8.0
- Dissolved oxygen:
- 4.7-5.5 mg/l (Start of test)
3.0-3.8 mg/l (End of test) - Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Test concentrations (mg/l) : 100, 1000 & 10000 (nominal)
- Details on test conditions:
- Test concentrations (mg/l): 100, 1000 & 10000
** Test item concentration in physico-chemical oxygen consumption control (mg/l) : 10000
Concentration reference substance 3.5-Dichlorophenol (mg/l): 5, 10 & 20
The test item and reference substance concentrations are not confirmed by analytical methods; they are based on nominal concentrations.
Type of application: direct weighing
Test concentration of the activated sludge: 960 mg/l suspended solids
Test temperature: 20 ± 2° C
Stirring period of test item before start of incubation time: 16 hours
Incubation time: 3 hours with permanent aeration
The physico-chemical oxygen consumption control is carried out, since some substances may consume oxygen by chemical reactivity. In order to be able to differentiate between physico-chemical oxygen consumption and biological oxygen consumption (respiration), at least the maximum concentration of the test item is additionally tested without activated sludge. - Reference substance (positive control):
- yes
- Remarks:
- 3.5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The substance showed 0.0% inhibition of the sewage sludge at a concentration of 10000 mg/l
- Results with reference substance (positive control):
- EC5o of the reference substance 3.5-dichlorophenol is in the range 5 - 30 mg/l
- Reported statistics and error estimates:
- All validity criteria of the test method were met:
respiratory rates of the 2 controls differ less than 15 %
respiratory rate of the controls < 60 mg 02 /l - h - Validity criteria fulfilled:
- yes
- Conclusions:
- Gelb Sulfato showed 0.0 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/l.
EC50> 10000 mg/l - Executive summary:
A study was performed to assess the toxicity of Gelb Sulfato to bacteria.
The study was conducted according to the Commission Directive 88/302/EEC; Part C.11 Activated Sludge Respiration Inhibition". This test method is in most parts identical with OECD Guideline 209"Activated Sludge, Respiration Inhibition Test". Study performed in accordance with the Principle of Good Laboratory Practice (GLP) and reported with a GLP certificate.
The activated sludge was exposed to Gelb 176 Sulfato at different concentrations. The respiration rate of each mixture was determined after aeration periods of 3 hours.
Gelb 176 Sulfato showed 0.0 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/l.
The EC50 is higher than 10000 mg/l.
The effect value related to nominal concentration, since no analytical monitoring was performed.
Reference
RESULTS
Test item:Gelb 176 Sulfato
Test concentration |
Respiratory rest test item |
Phys-chem. O2 consumption |
Respiratory rate – phys.-chem. O2 consumption |
Inhibition |
[mg/l] |
[mg/l – h] |
[mg/l – h] |
[mg/l – h] |
[%] |
100 |
34.0 |
*0.0 |
34.0 |
0.0 |
1000 |
34.0 |
*0.0 |
34.0 |
0.0 |
10000 |
36.0 |
0.0 |
36.0 |
0.0 |
Control. mean |
32.0 |
|
||
Control 1 |
30.0 |
|||
Control 2 |
34.0 |
Comments relevant to result: test conditions are given as nominal concentrations and have not been confirmed by analytical methods.
*The physico-chemical oxygen consumption has been determined at 10000 mg/l test item concentration. As no physic-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
Reference substance: 3.5-Dichlorophenol
Test concentration |
Respiratory rate |
Inhibition |
[mg/l] |
[mg/l – h] |
[%] |
5 |
24.0 |
25.0 |
10 |
20.0 |
37.5 |
20 |
7.2 |
77.5 |
Control. mean |
32.0 |
|
Control 1 |
30.0 |
|
Control 2 |
34.0 |
Comments: the reference substance concentrations result from the quantities used in the preparations and have not been confirmed by analytical methods.
Description of key information
Gelb 176 Sulfato (= Reactive Yellow 176 Ester) showed 0.0 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/l.
The EC50(3 h) is higher than 10000 mg/L test mat. based on respiration rate
Key value for chemical safety assessment
Additional information
A study was performed to assess the toxicity of Gelb 176 Sulfato (= Reactive Yellow 176 Ester) to bacteria. The study was conducted according to the Commission Directive 88/302/EEC; Part C.11 Activated Sludge Respiration Inhibition". This test method is in most parts identical with OECD Guideline 209"Activated Sludge, Respiration Inhibition Test".
The activated sludge was exposed to Gelb Sulfato at different concentrations. The respiration rate of each mixture was determined after aeration periods of 3 hours.
Gelb Sulfato showed 0.0 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/l.
The EC50 is higher than 10000 mg/l.
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