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EC number: 218-076-0 | CAS number: 2049-95-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 12 October 2007 to 11 April 2008.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 6.25, 12.5, 25, 50 and 100 % of a saturated stock solution.
- Sampling method: At test initiation bulk solutions were sampled. After 24 hours samples were taken from the test flasks in which all water fleas were immobile. After 48 hours samples were taken from all remaining test flasks.
- Sample storage conditions before analysis: 2-8°C. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: An amount of 200 mg tertiary-amylbenzene was dissolved in 2 litres Elendt M4 medium. This stock solution was continuously stirred and placed at a temperature of 20°C. On the day of test initiation the stock solution was filtered with filter paper (S&S 595 1/2) to remove undissolved
test substance.
- Controls: Controls with test medium were included.
- Evidence of undissolved material: No. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: Aquasense b.v., Amsterdam, The Netherlands
- Age at study initiation: 0 - 24 hours
- Method of breeding: Waterfleas are cultured in vessels with 2 liter spring water. They are fed with algal suspension of Chlorella vulgaris.
- Feeding during test: not applicable - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not applicable
- Hardness:
- No measurement.
- Test temperature:
- 19.8 - 20.9°C
- pH:
- Controls: 7.8 - 8.3
Test concentrations: 7.7 - 8.3 - Dissolved oxygen:
- Controls: 7.9 - 8.8 mg/l
Test concentrations: 7.7 - 8.8 mg/l - Nominal and measured concentrations:
- See Table 1.
- Details on test conditions:
- TEST SYSTEM
- Type: closed
- Material, size, headspace, fill volume: closed (glass) flasks with a volume of 250 ml were completely filled with test solution.
- Aeration: No.
- Renewal rate of test solution: No.
- No. of organisms per vessel: 10 water fleas.
- No. of vessels per concentration: 3 replicates.
- No. of vessels per control: 3 replicates.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium prepared from purified water.
- Culture medium different from test medium: No.
OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: 16 hours light/8 hours dark
EFFECT PARAMETERS MEASURED: The number of mobile and immobile water fleas and the number of water fleas showing sublethal effects was recorded 24 and 48 hours after test initiation. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Details on results:
- See Table 2.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the EC50/48h value based on immobility was 2.2 mg/L.
- Executive summary:
The acute toxicity of Tertiary-amylbenzene to water fleas (Daphnia magna) was tested following OECD Guideline 202 and according to GLP.
Based on a preliminary test the study was conducted at 0, 6.25, 12.5, 25, 50 and 100% of the filtrated stock solution. Because tertiary-amylbenzene will volatilise from the test solutions, the test was conducted in closed (glass) flasks with a volume of 250 mL. Three flasks, each completely filled with test solution, were prepared per concentration and 10 water fleas were added to each glass beaker. the water fleas were exposed for 48 hours to the test solutions.
It was demonstrated that the concentration of tertiary-amylbenzene has been maintained within 12.5% of the measured initial concentration throughout the test. The biological endpoints of the test are based on the average of the measured concentrations being 0, 0.62, 1.0, 2.0, 3.8 and 7.2 mg/L.
No immobility was observed in the control glass beakers. The No Observed Effect Concentration (NOEC) based on immobility was 1.0 mg/L. The EC50/48h value based on immobility was 2.2 mg/L.
Reference
Table 2: Immobility of Daphnia magna during the acute toxicity test
Mean measured concentration (mg/l) |
Number of immobile fleas |
Number of water fleas tested |
Percentage of immobility after 48 hours |
|
24 h |
48 h |
|||
n.d. |
0 |
0 |
30 |
0 |
0.62 |
0 |
0 |
30 |
0 |
1.0 |
0 |
0 |
30 |
0 |
2.0 |
5 |
5 |
30 |
16.7 |
3.8 |
30 |
30 |
30 |
100 |
7.2 |
30 |
30 |
30 |
100 |
Description of key information
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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