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EC number: 269-616-7 | CAS number: 68307-94-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The biodegradation of the test item was assessed according to OECD Guideline 301B. The test item attained 115% degradation after 28 days and satisfied the 10-Day window validation criterion, whereby 60% degradation must be attained within 10 days of the degradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301B.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Introduction.
A study was performed to assess the ready biodegradability of the test item in an aerobic aqueous medium. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (1992) No 301 B, "Ready Biodegradability; CO2 Evolution Test" referenced as Method C.4-C of Commission Regulation (EC) No. 440/2008 and US EPA Fate, Transport, and Transformation Test Guidelines OPPTS 835.3110 (Paragraph (M)).
Methods.
The test item, at a concentration of 10.3 mg Carbon/l, was exposed to activated sewage sludge micro-organisms with culture medium in sealed culture vessels in the dark at approximately 21°C for 28 days.
The degradation of the test item was assessed by the determination of carbon dioxide produced. Control solutions with inoculum and the reference item, sodium benzoate, together with a toxicity control were used for validation purposes.
Results.
The test item attained 115% degradation after 28 days and satisfied the 10-Day window validation criterion, whereby 60% degradation must be attained within 10 days of the degradation exceeding 10%. The test item can therefore be considered to be readily biodegradable under the strict terms and conditions of OECD Guideline No 301 B.
Degradation values in excess of 100% were considered to be due to an increase in the numbers of viable micro-organisms in the test item vessels as a result of the readily biodegradable nature of the test item. This effect occurs due to the micro-organisms utilizing the test item as a carbon source for cellular growth resulting in a greater number of viable micro-organisms in these vessels when compared to the control vessels. The increased number of micro-organisms in these vessels gave rise to increased respiration rates and hence background CO2evolution was greater than in the control vessels. This increase in background CO2 evolution resulted in biodegradation rates in excess of 100%.
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