Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 610-877-7 | CAS number: 52624-57-4
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- Oxirane, 2-methyl-, oligomeric reaction products with oxirane, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)
- EC Number:
- 610-877-7
- Cas Number:
- 52624-57-4
- Molecular formula:
- C6 H14 O3 . 3 (C3 H6 O . C2 H4 O)x
- IUPAC Name:
- Oxirane, 2-methyl-, oligomeric reaction products with oxirane, ether with 2-ethyl-2-(hydroxymethyl)-1,3-propanediol (3:1)
- Details on test material:
- - Name of test material (as cited in study report): Triol 9053
- Physical state: colourless-yellowish liquid
- Analytical purity: not reported
- Batch No.: Kreisprobe Tank 16
- Storage condition of test material: at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: Activated sludge from laboratory wastewater plants treating municipal sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated tor 24 hours.
50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test.
- Initial biomass concentration: 1 g/L
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.1 - 7.5
- Nominal and measured concentrations:
- 1000 mg/L (nominal)
- Details on test conditions:
- - Test vessel: Erlenmeyer-vessel
- Nominal volume: 250 ml
- Test volume: 250 ml
- Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrations
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- EC20 = ca. 1.8 mg/L
EC50 = ca 10 mg/L
EC80 = ca. 55 mg/L
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
